农杆菌介导的玉米转化研究进展

Advances in Agrobacterium-mediated Maize Transformation.

作者信息

Zhong Heng, Elumalai Sivamani, Nalapalli Samson, Richbourg Lee, Prairie Anna, Bradley David, Dong Shujie, Su Xiujuan Jenny, Gu Weining, Strebe Tim, Shi Liang, Que Qiudeng

机构信息

Syngenta Crop Protection, LLC, 9 Davis Drive, Research Triangle Park, Durham, NC, 27709, USA.

出版信息

Methods Mol Biol. 2018;1676:41-59. doi: 10.1007/978-1-4939-7315-6_3.

DOI:10.1007/978-1-4939-7315-6_3

PMID:28986903

Abstract

One of the major limitations of maize transformation is the isolation of a large number of immature embryos using the time-consuming manual extraction method. In this article, we describe a novel bulk embryo extraction method for fast isolation of a large number of embryos suitable for both biolistic- and Agrobacterium-mediated transformation. Optimal gene delivery and tissue culture conditions are also described for achieving high efficiency in Agrobacterium-mediated maize transformation using phosphomannose isomerase (PMI) as a selectable marker.

摘要

玉米转化的主要限制之一是使用耗时的手工提取方法分离大量未成熟胚。在本文中，我们描述了一种新型的批量胚提取方法，用于快速分离大量适用于生物弹道法和农杆菌介导转化的胚。还描述了以磷酸甘露糖异构酶（PMI）作为选择标记，在农杆菌介导的玉米转化中实现高效转化的最佳基因导入和组织培养条件。