利用高空间分辨率基质辅助激光解吸电离质谱成像技术对玉米脂质和代谢物进行细胞及亚细胞水平定位

Cellular and Subcellular Level Localization of Maize Lipids and Metabolites Using High-Spatial Resolution MALDI Mass Spectrometry Imaging.

作者信息

Dueñas Maria Emilia, Feenstra Adam D, Korte Andrew R, Hinners Paige, Lee Young Jin

机构信息

Department of Chemistry, Iowa State University of Science and Technology, 35A Carver Co-Lab, 1111 Woi Rd., Ames, IA, 50011, USA.

Ames Laboratory, U.S. Department of Energy, Ames, IA, 50011, USA.

出版信息

Methods Mol Biol. 2018;1676:217-231. doi: 10.1007/978-1-4939-7315-6_13.

DOI:10.1007/978-1-4939-7315-6_13

PMID:28986913

Abstract

Recent technological advances have pushed the achievable spatial resolution for mass spectrometry imaging (MSI) to cellular and subcellular levels. Direct visualization of maize tissues by this tool has provided key insights into the localization of metabolites and lipids. This chapter outlines methodology for sample preparation, data acquisition, and data analysis of maize tissue sections using high-spatial resolution matrix-assisted laser desorption ionization (MALDI)-MSI, as well as the incorporation of a multi-resolution optical system, which allows for simple inter-conversion between different resolution setups (5, 10, and 50 μm imaging).

摘要

最近的技术进步已将质谱成像（MSI）可实现的空间分辨率提升至细胞和亚细胞水平。利用该工具对玉米组织进行直接可视化，为代谢物和脂质的定位提供了关键见解。本章概述了使用高空间分辨率基质辅助激光解吸电离（MALDI）-MSI对玉米组织切片进行样品制备、数据采集和数据分析的方法，以及多分辨率光学系统的应用，该系统允许在不同分辨率设置（5、10和50μm成像）之间进行简单的相互转换。