Sun Peifang, Crum-Cianflone Nancy F, Defang Gabriel, Williams Maya, Ganesan Anuradha, Agan Brian K, Lalani Tahaniyat, Whitman Timothy, Brandt Carolyn, Burgess Timothy H
Henry Jackson Foundation, United States.
Naval Medical Center, San Diego, CA, United States.
Vaccine. 2017 Oct 27;35(45):6103-6111. doi: 10.1016/j.vaccine.2017.09.059. Epub 2017 Oct 4.
This study was to compare B and T memory cells elicited by a single dose monovalent 2009 influenza A (H1N1) vaccine (strain A/California/7/2009 H1N1) in HIV and HIV groups, and to analyze the impact of the prior seasonal vaccines to the immunogenicity of this vaccine.
Blood samples were collected before vaccination (day 0) and at days 28 and 180. Participants were categorized into HIV/LAIV, HIV/TIV and HIV/TIV subgroups according to the trivalent live-attenuated or inactivated (LAIV or TIV) seasonal influenza vaccines they received previously. The IgG memory B cells (B) and IFNγ T cells were measured against antigens including the H1N1 vaccine, the hemagglutinin (HA) and neuraminidase (NA) proteins or peptide pools of the pandemic and the seasonal H1N1 strains, respectively.
Overall B responses increased significantly at day 28 but returned to baseline by day 180 in all three subgroups. The average frequency of the H1N1-specific B at day 28 for the HIV/LAIV, HIV/TIV and HIV/TIV groups was 2.14%, 1.26% and 1.67%, respectively, and the average fold change was 14.39, 3.81 and 3.93, respectively. The differences of B between HIV/LAIV and the two TIV subgroups were significant. For the IFNγ response, the overall spot counts ranged widely between 0 and 958/10 PBMCs. The group average spot counts to H1N1 vaccine was 89, 102, and 30 at day 28 for HIV/LAIV, HIV/TIV and HIV/TIV subgroups, respectively. The average increase of IFNγ response at day 28 vs day 0 in all three subgroups did not reach 2-fold.
Participants with a prior LAIV seasonal vaccine, as compared to a TIV seasonal vaccine, responded significantly better to the monovalent H1N1 vaccine. Excluding LAIV participants, no difference was seen between the HIV and HIV subject groups in terms of B. The B response declined at 6months.
本研究旨在比较单剂量单价2009甲型H1N1流感疫苗（A/加利福尼亚/7/2009 H1N1毒株）在HIV感染者和非HIV感染者中引发的B细胞和T细胞记忆反应，并分析既往季节性疫苗对该疫苗免疫原性的影响。
在接种疫苗前（第0天）以及第28天和第180天采集血样。根据参与者之前接种的三价减毒活疫苗或灭活疫苗（LAIV或TIV），将其分为HIV/LAIV组、HIV/TIV组和HIV/TIV亚组。分别针对包括H1N1疫苗、大流行和季节性H1N1毒株的血凝素（HA）和神经氨酸酶（NA）蛋白或肽库等抗原，检测IgG记忆B细胞（B细胞）和IFNγ T细胞。
在所有三个亚组中，总体B细胞反应在第28天显著增加，但在第180天恢复到基线水平。HIV/LAIV组、HIV/TIV组和HIV/TIV亚组在第28天H1N1特异性B细胞的平均频率分别为2.14%、1.26%和1.67%，平均变化倍数分别为14.39、3.81和3.93。HIV/LAIV组与两个TIV亚组之间的B细胞反应差异显著。对于IFNγ反应，总体斑点计数在0至958/10个外周血单个核细胞之间广泛分布。HIV/LAIV组、HIV/TIV组和HIV/TIV亚组在第28天对H1N1疫苗的组平均斑点计数分别为89、102和30。所有三个亚组在第28天相对于第0天的IFNγ反应平均增加未达到2倍。
与接种TIV季节性疫苗的参与者相比，既往接种LAIV季节性疫苗的参与者对单价H1N1疫苗的反应明显更好。排除LAIV参与者后，HIV感染者和非HIV感染者在B细胞反应方面没有差异。B细胞反应在6个月时下降。
