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[细菌基因组片段可能进入痢疾变形虫滋养体细胞核]

[Possible entry of fragments of a bacterial genome into the trophozoite nucleus of the dysentery amoeba].

作者信息

Chernov Iu V, Prodeus T V, Gordeeva L M

出版信息

Parazitologiia. 1988 Mar-Apr;22(2):149-53.

PMID:2898747
Abstract

The use of Fölgen-like electron microscopy reaction has revealed particles in the cytoplasm and nucleus of the dysentery amoeba trophozoites which in their size and resistance to acidic hydrolysis and in their affinity for uranilacetate are similar to tightly packed elements of the bacterial genome. On this basis a conclusion was drawn that some fragments of genomes of phagocytic bacteria can be preserved in the amoebal cell. The community of microorganisms consisting of trophozoites of the dysentery amoeba and bacteria of the intestinal flora is supposed to be a suitable model for studying the possibility of penetration of transgenetic elements from procaryote to eucaryote.

摘要

使用类似福尔根的电子显微镜反应已揭示,痢疾变形虫滋养体的细胞质和细胞核中存在颗粒,这些颗粒在大小、对酸性水解的抗性以及对醋酸双氧铀的亲和力方面,与紧密排列的细菌基因组元件相似。基于此得出结论,吞噬细菌基因组的一些片段可在变形虫细胞中保存。由痢疾变形虫滋养体和肠道菌群细菌组成的微生物群落被认为是研究转基因元件从原核生物渗透到真核生物可能性的合适模型。

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