血清白细胞介素检测可能有助于识别患有活动性疾病的甲状腺癌患者。

Serum interleukin measurement may help identify thyroid cancer patients with active disease.

作者信息

Martins Mariana Bonjiorno, Marcello Marjory Alana, Batista Fernando de Assis, Peres Karina Colombera, Meneghetti Murilo, Ward Mirela Andrea Latham, Etchebehere Elba Cristina Sá de Camargo, da Assumpção Ligia Vera Montali, Ward Laura Sterian

机构信息

Laboratory of Cancer Molecular Genetics, Faculty of Medical Sciences (FCM), University of Campinas (Unicamp), 126, Tessalia Vieira de Camargo St., Campinas, SP, Brazil.

出版信息

Clin Biochem. 2018 Feb;52:1-7. doi: 10.1016/j.clinbiochem.2017.10.003. Epub 2017 Oct 5.

DOI:10.1016/j.clinbiochem.2017.10.003

PMID:28987791

Abstract

BACKGROUND

Investigate the clinical utility of serum interleukin dosages of IL-2, IL-2R, IL-4, IL-6, IL-6R, IL-8, IL-10 and IL-12 in the diagnosis and characterization of patients with DTC. In particular, verify ILs utility in the identification of individuals who are evolving disease-free or with the active disease.

METHODS

We evaluated 200 patients with malignant nodules (100 patients disease-free and 100 patients with recurrence/active disease); 60 benign nodules and 100 healthy controls, serum levels were assessed by ELISA.

RESULTS

All ILs, but not IL-4, differentiated these three groups. We observed that IL-2, 2R and 10 serum concentrations were associated with thyroglobulin levels. Serum IL-2 was able to differentiate patients with active disease from the disease-free with a sensitivity of 98%, specificity of 58%, positive predictive value (PPV) of 70% and negative predictive value (NPV) of 97% (p=0.0007). IL-6R levels differentiated patients with active disease from the disease-free patients with 56% sensitivity, 63% specificity, PPV of 60% and NPV of 59% (p<0.0001). IL-8 values also distinguished patients with active disease from the disease-free ones with sensitivity of 50%, specificity of 76%, PPV of 68% and NPV of 60% (p=0.0025); using IL-12, we obtained a sensitivity value of 73%, specificity of 66%, PPV of 68% and NPV of 71% (p<0.0001). Furthermore, interleukin levels showed association with some tumor characteristics of aggressiveness.

CONCLUSION

We suggest that the serum concentration of ILs may assist in the diagnosis and characterization of tumor malignancy helping identify patients with active disease who deserve closer medical attention.

摘要

背景

研究血清白细胞介素（IL）-2、IL-2受体（IL-2R）、IL-4、IL-6、IL-6R、IL-8、IL-10和IL-12的剂量在分化型甲状腺癌（DTC）患者诊断及特征描述中的临床应用。尤其要验证白细胞介素在识别病情无进展或患有活动性疾病个体方面的效用。

方法

我们评估了200例恶性结节患者（100例病情无进展患者和100例复发/活动性疾病患者）；60例良性结节患者和100例健康对照者，通过酶联免疫吸附测定（ELISA）评估血清水平。

结果

除IL-4外，所有白细胞介素均可区分这三组。我们观察到IL-2、IL-2R和IL-10的血清浓度与甲状腺球蛋白水平相关。血清IL-2能够区分活动性疾病患者和病情无进展患者，敏感性为98%，特异性为58%，阳性预测值（PPV）为70%，阴性预测值（NPV）为97%（p = 0.0007）。IL-6R水平区分活动性疾病患者和病情无进展患者，敏感性为56%，特异性为63%，PPV为60%，NPV为59%（p < 0.0001）。IL-8值也可区分活动性疾病患者和病情无进展患者，敏感性为50%，特异性为76%，PPV为68%，NPV为60%（p = 0.0025）；使用IL-12时，我们得到的敏感性值为73%，特异性为66%，PPV为68%，NPV为71%（p < 0.0001）。此外，白细胞介素水平显示出与一些侵袭性肿瘤特征相关。