Kist Tarso B Ledur
Laboratory of Methods, Department of Biophysics, Institute of Biosciences, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
J Sep Sci. 2017 Dec;40(23):4619-4627. doi: 10.1002/jssc.201700712. Epub 2017 Nov 13.
The ability of a method and instrument to separate very similar compounds is related to the "plate number," a number indicating performance. The resolution between two neighboring peaks is proportional to the square root of the plate number. Currently available commercial capillary electrophoresis instruments easily reach plate numbers of a few million. In the present work, a capillary electrophoresis system with a toroidal platform is proposed and theoretically studied with the goal of extending the achievable plate number. In this new system, electrophoresis occurs in a nonstop continuous circulating mode within a closed loop capillary (toroid). Plate numbers upwards of one billion are theoretically predicted. This could resolve hundreds of unseparated mixtures of stereoisomers and other analytes that remain without a method for their analysis.
一种方法和仪器分离非常相似化合物的能力与“塔板数”相关，塔板数是一个表示性能的数值。相邻两个峰之间的分辨率与塔板数的平方根成正比。目前市面上的商用毛细管电泳仪器很容易达到数百万的塔板数。在本研究中，提出了一种带有环形平台的毛细管电泳系统，并对其进行了理论研究，目的是提高可达到的塔板数。在这个新系统中，电泳在一个闭环毛细管（环形）内以不间断的连续循环模式进行。理论预测塔板数可超过十亿。这可以分离数百种尚未分离的立体异构体混合物和其他分析物，而目前尚无分析它们的方法。
