Kumari Reena, Kumar Sandeep, Ahmad M Kaleem, Singh Rajeev, Pradhan Akshayaya, Chandra Sharad, Kumar Sudhir
Department of Biochemistry, KGMU, Lucknow 226003, India; Department of Zoology, University of Lucknow, Lucknow 226007, India.
Department of Clinical Immunology, SGPGIMS, Lucknow 226014, India.
Diabetes Metab Syndr. 2018 May;12(3):221-225. doi: 10.1016/j.dsx.2017.09.006. Epub 2017 Sep 22.
BACKGROUND/AIM: Cytokines are responsible for the modulation of immunological and inflammatory processes and play a significant role in the pathogenesis of coronary artery disease (CAD). Tumor necrosis factor-alpha (TNF-α) are considered as a pro inflammatory and interleukin-10 (IL-10) anti inflammatory have been shown to predict the risk of incident of CAD. Aim of present study is to examine the impact of the TNF-α and Il-10 levels on various components of the CAD.
Total 580 subjects were recruited in the present study out of which 290 diagnosed CAD subjects (Age 51.61±9.26; BMI 25.27±3.58) and 290 healthy controls (Age 51.72±9.48; BMI 24.02±7.42). Serum TNF-alpha and IL-10 levels were measured by ELISA.
Data of present study revealed that CAD patients had higher frequency (p=0.001) of smoking (38.28%), Alcohol (21.03%), Diabetes (53.45%) and hypertension (48.28%) as compare to controls. Moreover, we have observed highly significant (p<0.05) difference in PR, BMI, DBP, DBP, Glucose, TC, TG, VLDL, LDL, TNF-α and TNF-α/IL-10. However, HDL and IL-10 were found lower in CAD.
The findings of present study suggest that the TNF-α/IL-10 ratio may play a vital role in the development of CAD of North Indian population.
背景/目的：细胞因子负责调节免疫和炎症过程，在冠状动脉疾病（CAD）的发病机制中起重要作用。肿瘤坏死因子-α（TNF-α）被认为是促炎因子，而白细胞介素-10（IL-10）是抗炎因子，它们已被证明可预测CAD发病风险。本研究的目的是探讨TNF-α和IL-10水平对CAD各组成部分的影响。
本研究共招募了580名受试者，其中290名被诊断为CAD的受试者（年龄51.61±9.26；体重指数25.27±3.58）和290名健康对照者（年龄51.72±9.48；体重指数24.02±7.42）。采用酶联免疫吸附测定法（ELISA）检测血清TNF-α和IL-10水平。
本研究数据显示，与对照组相比，CAD患者吸烟（38.28%）、饮酒（21.03%）、糖尿病（53.45%）和高血压（48.28%）的发生率更高（p=0.001）。此外，我们观察到PR、体重指数、舒张压、舒张压、血糖、总胆固醇、甘油三酯、极低密度脂蛋白、低密度脂蛋白、TNF-α和TNF-α/IL-10存在高度显著差异（p<0.05）。然而，CAD患者的高密度脂蛋白和IL-10水平较低。
本研究结果表明，TNF-α/IL-10比值可能在北印度人群CAD的发生发展中起重要作用。
