College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, China.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, China.
Exp Gerontol. 2017 Dec 15;100:36-44. doi: 10.1016/j.exger.2017.09.022. Epub 2017 Oct 6.
The aggregated lymphoid nodules area (ALNA) in the abomasum is a special organized lymphoid tissue discovered only in Bactrian camels at present. This study aimed to explore the impact of aging on distribution of IgA and IgG cells in ALNA in abomasum of Bactrian camels. Twenty-four Alashan Bactrian camels were divided into the following four age groups: young (1-2years), pubertal (3-5years), middle-aged (6-16years) and old (17-20years). IgA and IgG cells in the lamina propria of ALNA were observed and analyzed using immunohistochemical and statistical techniques. The results showed that, in ALNA, the distribution of IgA and IgG cells were diffuse, and only a few were in subepithelium dome (SED) and most of them in non-SED. Meanwhile, there were significantly more IgA cells than IgG cells in SED from the young to the middle aged group, but which reversed in old group (P<0.05). However, the aging significantly decreased the densities of IgA and IgG cells populations in non-SED (P<0.05); in SED, there were no significant differences between the densities of IgA and IgG cells, but which were both significantly lower in old group than those in young group (P<0.05). The results demonstrated that, in mucosal effector sites, the aging significantly decreased the densities of IgA and IgG cells populations and impacted on the defense barriers formed by IgA and IgG, but had no impact on the scattered characteristics. In inductive sites, the aging dramatically declined their densities, and they should have close relationships with immune memory. These findings lay the foundation for further researching the mucosal immune disorder or decline caused by aging, and especially underscore the importance of researching the impact of aging on the relationship between IgA and IgG cells populations and the microbiota colonized in abomasum of Bactrian camels.
皱胃集合淋巴滤泡区（ALNA）是目前仅在双峰驼中发现的一种特殊的组织化淋巴组织。本研究旨在探讨年龄对双峰驼皱胃 ALNA 中 IgA 和 IgG 细胞分布的影响。将 24 峰阿拉善双峰驼分为以下四个年龄组：青年组（1-2 岁）、性成熟组（3-5 岁）、中年组（6-16 岁）和老年组（17-20 岁）。采用免疫组织化学和统计学技术观察和分析 ALNA 固有层中 IgA 和 IgG 细胞的分布。结果表明，在 ALNA 中，IgA 和 IgG 细胞的分布呈弥漫性，仅少数位于上皮下穹窿（SED），大多数位于非-SED。同时，从青年到中年组，SED 中 IgA 细胞明显多于 IgG 细胞，但老年组则相反（P<0.05）。然而，老化显著降低了非-SED 中 IgA 和 IgG 细胞群体的密度（P<0.05）；SED 中，IgA 和 IgG 细胞的密度没有差异，但老年组均显著低于青年组（P<0.05）。结果表明，在黏膜效应部位，老化显著降低了 IgA 和 IgG 细胞群体的密度，并影响了 IgA 和 IgG 形成的防御屏障，但对其分散特征没有影响。在诱导部位，老化显著降低了它们的密度，它们与免疫记忆可能有密切关系。这些发现为进一步研究衰老引起的黏膜免疫紊乱或下降奠定了基础，特别是强调了研究衰老对双峰驼皱胃 IgA 和 IgG 细胞群体与定居菌群之间关系的影响的重要性。
