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[肠炎沙门氏菌中钾离子摄取的特征]

[Characteristics of K+ uptake in S. enteritidis bacteria].

作者信息

Ter-Nikogosian V A, Trchunian A A, Martirosov S M

出版信息

Biofizika. 1988 Mar-Apr;33(2):310-3.

PMID:2898951
Abstract

Bacterial Salmonella enteritidis var. Issatchenko in media without exogenic energy source uptakes K+ in one step with Km 2.1 mM and Vmax 0.08 mM min-1/10(12) cells. This K+ uptake does not depend on pH and osmotic shock and is not inhibited by DCC. Endogenic energy source (glucose) leads to K+ uptake with Km 2.8 mM and Vmax 0.10 mM min-1/10(12) cells, and secretion of H+. The ratio of the DCC-sensitive fluxes of H+ to K+ equals 2. Arsenate and protonophores depress the K+ uptake. Valinomycin decreases the rate of K+ uptake. It is assumed that K+ uptake takes place via the Trk-like system, which works as a separate system as supercomplex with the H+-ATPase complex.

摘要

肠炎沙门氏菌伊萨琴科变种在没有外源性能源的培养基中,以Km为2.1 mM、Vmax为0.08 mM min⁻¹/10¹²个细胞的速率一步摄取钾离子。这种钾离子摄取不依赖于pH值和渗透压休克,也不受二环己基碳二亚胺(DCC)抑制。内源性能源(葡萄糖)导致以Km为2.8 mM、Vmax为0.10 mM min⁻¹/10¹²个细胞的速率摄取钾离子,并分泌氢离子。氢离子与钾离子对DCC敏感的通量之比为2。砷酸盐和质子载体抑制钾离子摄取。缬氨霉素降低钾离子摄取速率。据推测,钾离子摄取是通过类Trk系统进行的,该系统作为与H⁺-ATP酶复合体的超复合体的一个独立系统发挥作用。

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