列当属及其在列当科中的应用：II. 单拷贝核基因座的引物
Primers for and their utility across Orobanchaceae: II. Single-copy nuclear loci.
作者信息
Latvis Maribeth, Jacobs Sarah J, Mortimer Sebastian M E, Richards Melissa, Blischak Paul D, Mathews Sarah, Tank David C
机构信息
Department of Natural Resource Management, South Dakota State University, 1390 College Avenue, Brookings, South Dakota 57007 USA.
Department of Biological Sciences, University of Idaho, 875 Perimeter Drive, MS 3051, Moscow, Idaho 83844-3051 USA.
出版信息
Appl Plant Sci. 2017 Sep 30;5(9). doi: 10.3732/apps.1700038. eCollection 2017 Sep.
PREMISE OF THE STUDY
We developed primers targeting nuclear loci in with the goal of reconstructing the evolutionary history of this challenging clade. These primers were tested across other major clades in Orobanchaceae to assess their broader utility.
METHODS AND RESULTS
We assembled low-coverage genomes for three taxa in and developed primer combinations for the single-copy conserved ortholog set (COSII) and the pentatricopeptide repeat (PPR) gene family. These primer combinations were designed to take advantage of the Fluidigm microfluidic PCR platform and are well suited for high-throughput sequencing applications. Eighty-seven primers were designed for , and 27 were found to have broader utility in Orobanchaceae.
CONCLUSIONS
These results demonstrate the utility of these primers, not only across , but for other lineages within Orobanchaceae as well. This expanded molecular toolkit will be an asset to future phylogenetic studies in and throughout Orobanchaceae.
研究前提
我们开发了针对[具体植物名称]核基因座的引物，目的是重建这个具有挑战性的分支的进化历史。这些引物在列当科的其他主要分支中进行了测试，以评估其更广泛的实用性。
方法与结果
我们为[具体植物名称]的三个分类单元组装了低覆盖度基因组，并为单拷贝保守直系同源集（COSII）和五肽重复序列（PPR）基因家族开发了引物组合。这些引物组合旨在利用Fluidigm微流控PCR平台，非常适合高通量测序应用。我们为[具体植物名称]设计了87个引物，发现其中27个在列当科具有更广泛的实用性。
结论
这些结果证明了这些引物的实用性，不仅适用于[具体植物名称]，也适用于列当科的其他谱系。这个扩展的分子工具包将有助于未来对[具体植物名称]和整个列当科的系统发育研究。
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