Mocanu S, Matei I, Ionescu S, Tecuceanu V, Marinescu G, Ionita P, Culita D, Leonties A, Ionita Gabriela
"Ilie Murgulescu" Institute of Physical Chemistry of the Romanian Academy, 202 Splaiul Independentei, Bucharest 060021, Romania.
Phys Chem Chem Phys. 2017 Oct 18;19(40):27839-27847. doi: 10.1039/c7cp05276f.
Electron paramagnetic resonance (EPR) and fluorescence spectroscopies provide molecular-level insights on the interaction of paramagnetic and fluorescent species with the microenvironment. A series of dual molecular probes bearing fluorescent and paramagnetic moieties linked by flexible short polyether chains have been synthesized. These new molecular probes open the possibility to investigate various multi-component systems such as host-guest systems, polymeric micelles, gels and protein solutions by using EPR and fluorescence spectroscopies concertedly. The EPR and fluorescence spectra of these compounds show that the dependence of the rotational correlation time and fluorescence quantum yield on the chain length of the linker is not linear, due to the flexibility of the polyether linker. The quenching effect of the nitroxide moiety on the fluorescence intensity of the pyrene group varies with the linker length and flexibility. The interaction of these dual molecular probes with β-cyclodextrin, in solution and in polymeric gels, was evaluated and demonstrated by analysis of EPR and fluorescence spectra.
电子顺磁共振（EPR）和荧光光谱能够从分子层面深入了解顺磁性和荧光物质与微环境的相互作用。一系列带有通过柔性短聚醚链相连的荧光和顺磁性部分的双分子探针已被合成。这些新型分子探针为协同使用EPR和荧光光谱研究各种多组分体系（如主客体体系、聚合物胶束、凝胶和蛋白质溶液）开辟了可能性。这些化合物的EPR和荧光光谱表明，由于聚醚连接基的灵活性，旋转相关时间和荧光量子产率对连接基链长的依赖性并非呈线性关系。氮氧自由基部分对芘基团荧光强度的猝灭效应随连接基长度和灵活性而变化。通过对EPR和荧光光谱的分析，评估并证明了这些双分子探针在溶液和聚合物凝胶中与β-环糊精的相互作用。
