Chow S, Kurogi H, Yamamoto T, Tomoda T, Mochioka N, Shirotori F, Yoshinaga T, Ambe D, Okazaki M, Nagai S, Yanagimoto T
National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, 2-12-4 Fukuura, Yokohama, Kanagawa, 236-8648, Japan.
Yokosuka Laboratory, National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, 6-3-1, Nagai, Kanagawa, 238-0316, Japan.
J Fish Biol. 2017 Nov;91(5):1517-1525. doi: 10.1111/jfb.13483. Epub 2017 Oct 9.
Species-specific restriction fragment length polymorphism in the intron of the androgen receptor gene (ar5) was found in glass to silver-stage individuals of Anguilla japonica (n = 51) and A. marmorata (n = 21). The sequence analysis of 16S rDNA from 328 anguillid leptocephali collected in the North Equatorial Current of the western North Pacific Ocean revealed the specimens to be A. japonica (n = 194), A. marmorata (n = 128), A. bicolor pacifica (n = 5) and A. luzonensis (n = 1). All leptocephali of A. japonica and A. marmorata were monomorphic and did not share an allele at the ar5 locus, indicating that the two species are reproductively isolated.
在日本鳗鲡（n = 51）和云纹鳗鲡（n = 21）玻璃鳗至银鳗阶段的个体中，发现了雄激素受体基因内含子（ar5）的物种特异性限制性片段长度多态性。对在北太平洋西部赤道洋流采集的328尾鳗鲡柳叶鳗的16S rDNA进行序列分析，结果显示样本为日本鳗鲡（n = 194）、云纹鳗鲡（n = 128）、太平洋双色鳗鲡（n = 5）和吕宋鳗鲡（n = 1）。所有日本鳗鲡和云纹鳗鲡的柳叶鳗都是单态的，在ar5位点不共享等位基因，这表明这两个物种在生殖上是隔离的。
