Research Institute of Experimental and Clinical Medicine , 2 Timakova st., 630117 Novosibirsk, Russian Federation.
Research Institute of Clinical and Experimental Lymphology-Branch of the ICG SB RAS , 2 Timakova st., 630060 Novosibirsk, Russian Federation.
Inorg Chem. 2017 Nov 6;56(21):13491-13499. doi: 10.1021/acs.inorgchem.7b02212. Epub 2017 Oct 9.
Octahedral rhenium cluster complexes have recently emerged as relevant building blocks for the design of singlet oxygen photosensitizing materials toward biological applications such as blue-light photodynamic therapy. However, their singlet oxygen generation ability as well as biological properties have been studied only superficially. Herein we investigate in detail the singlet oxygen photogeneration, dark and photoinduced cytotoxicity, cellular uptake kinetics, cellular localization and in vitro photoinduced oxidative stress, and photodynamic cytotoxicity of the series of octahedral rhenium cluster complexes [{ReQ}(CN)], where Q = S, Se, Te. Our results demonstrate that the selenium-containing complex possesses optimal properties in terms of absorption and singlet oxygen productivity. These features coupled with the cellular internalization and low dark toxicity lead to the first photoinduced cytotoxic effect observed for a molecular [{MQ}L] complex, making it a promising object for further study in terms of blue-light PDT.
八面体铼簇配合物最近作为设计单线态氧光敏材料的相关构建块出现，用于生物应用，如蓝光光动力疗法。然而，它们的单线态氧生成能力以及生物学特性仅被初步研究。在此，我们详细研究了系列八面体铼簇配合物 [{ReQ}(CN)] 的单线态氧光生成、暗毒性和光诱导细胞毒性、细胞摄取动力学、细胞定位和体外光诱导氧化应激以及光动力细胞毒性，其中 Q = S、Se、Te。我们的结果表明，含硒的配合物在吸收和单线态氧生成方面具有最佳性能。这些特性加上细胞内化和低暗毒性导致了首个观察到的分子 [{MQ}L] 配合物的光诱导细胞毒性效应，使其成为进一步研究蓝光 PDT 的有前途的对象。
