National Pediatric Myoclonus Center, National Pediatric Neuroinflammation Organization, Inc., Orlando, FL, USA.
J Neurol Sci. 2017 Oct 15;381:285-290. doi: 10.1016/j.jns.2017.09.006. Epub 2017 Sep 6.
To assess the role of microglia and macrophages in neuroinflammatory disorders in children via biomarkers, and establish control reference ranges.
In an IRB-approved case-control study of 98 children, the concentrations of CSF/serum CHI3L1, sCD14, and sCD163 were measured by ELISA. Groups were controls (non-inflammatory neurological disorders, NIND, n=37), opsoclonus-myoclonus syndrome (OMS, n=37), and other inflammatory neurological disorders (OIND, n=24).
In control CSF, median concentrations (ng/ml) were 25 (IQR 16,41) for CHI3L1 and 42 (26,160) for sCD14; in serum, 16 (12,22) for CHI3L1, and 431 (270,957) for sCD163. The median CSF concentration of CHI3L1 in OIND was significantly higher than controls (2.9-fold, P<0.0001) and OMS (1.6-fold higher than controls, NS). The CSF sCD14 concentration was 1.9-fold higher in OIND (P=0.008) and 1.4-fold higher in OMS than controls. sCD163, below detection limits in CSF, was not significantly increased in OIND or OMS sera.
CSF CHI3L1 and sCD14 elevations hold promise as immunomarkers in pediatric OIND, especially in high-expression individuals. These results provide evidence of innate immune system involvement in several pediatric neuroinflammatory disorders. Pediatric control data on CSF microglia/macrophage activation markers are hereby available for other investigators.
通过生物标志物评估儿童神经炎症性疾病中小胶质细胞和巨噬细胞的作用，并建立对照参考范围。
在一项经机构审查委员会批准的 98 例儿童病例对照研究中，通过 ELISA 法测定 CSF/血清 CHI3L1、sCD14 和 sCD163 的浓度。对照组（非炎症性神经疾病，NIND，n=37）、眼-面-肌阵挛-共济失调综合征（OMS，n=37）和其他炎症性神经疾病（OIND，n=24）。
在对照组的 CSF 中，CHI3L1 的中位数浓度（ng/ml）为 25（16，41），sCD14 为 42（26，160）；血清中，CHI3L1 为 16（12，22），sCD163 为 431（270，957）。OIND 患者的 CSF CHI3L1 中位数浓度明显高于对照组（2.9 倍，P<0.0001）和 OMS（比对照组高 1.6 倍，NS）。OIND 的 CSF sCD14 浓度高 1.9 倍（P=0.008），高于对照组 1.4 倍。sCD163 在 CSF 中检测不到，在 OIND 或 OMS 血清中也没有明显升高。
CSF CHI3L1 和 sCD14 的升高有望成为儿科 OIND 的免疫标志物，尤其是在高表达个体中。这些结果为几种儿科神经炎症性疾病中固有免疫系统的参与提供了证据。现在可为其他研究人员提供儿科 CSF 小胶质细胞/巨噬细胞激活标志物的对照数据。
