School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
Plant Physiol Biochem. 2017 Nov;120:95-102. doi: 10.1016/j.plaphy.2017.09.021. Epub 2017 Sep 28.
Benzene, a carcinogenic compound, has been reported as a major indoor air pollutant. Chlorophytum comosum (C. comosum) was reported to be the highest efficient benzene removal plant among other screened plants. Our previous studies found that plants under light conditions could remove gaseous benzene higher than under dark conditions. Therefore, C. comosum exposure to airborne benzene was studied under different light quality at the same light intensity. C. comosum could remove 500 ppm gaseous benzene with the highest efficiency of 68.77% under Blue:Red = 1:1 LED treatments and the lowest one appeared 57.41% under white fluorescent treatment within 8 days. After benzene was uptaken by C. comosum, benzene was oxidized to be phenol in the plant cells by cytochrome P450 monooxygenase system. Then, phenol was catalyzed to be catechol that was confirmed by the up-regulation of phenol 2-monooxygenase (PMO) gene expression. After that, catechol was changed to cic, cis-muconic acid. Interestingly, cis,cis-muconic acid production was found in the plant tissues higher than phenol and catechol. The result confirmed that NADPH-cytochrome P450 reductase (CPR), cytochrome b5 (cyt b5), phenol 2-monooxygenase (PMO) and cytochrome P450 90B1 (CYP90B1) in plant cells were involved in benzene degradation or detoxification. In addition, phenol, catechol, and cis,cis-muconic acid production were found under the Blue-Red LED light conditions higher than under white fluorescent light conditions due to under LED light conditions gave higher NADPH contents. Hence, C. comosum under the Blue-Red LED light conditions had a high potential to remove benzene in a contaminated site.
苯是一种致癌化合物，已被报道为主要的室内空气污染物。绿宝石（Chlorophytum comosum）被报道为在其他筛选的植物中去除苯的效率最高的植物。我们之前的研究发现，在光照条件下，植物去除气态苯的效率高于黑暗条件下。因此，在相同光照强度下，研究了空气中苯暴露在不同光质下对绿宝石的影响。在 8 天内，绿宝石在蓝：红=1:1 LED 处理下以 68.77%的最高效率去除 500 ppm 的气态苯，而在白色荧光处理下的最低去除率为 57.41%。苯被绿宝石吸收后，细胞中的细胞色素 P450 单加氧酶系统将其氧化为苯酚。然后，苯酚被催化为儿茶酚，这一点通过酚 2-单加氧酶（PMO）基因表达的上调得到证实。之后，儿茶酚转化为 cic，顺式粘酸。有趣的是，在植物组织中发现顺式，顺式粘酸的生成量高于苯酚和儿茶酚。该结果证实植物细胞中的 NADPH-细胞色素 P450 还原酶（CPR）、细胞色素 b5（cyt b5）、酚 2-单加氧酶（PMO）和细胞色素 P450 90B1（CYP90B1）参与了苯的降解或解毒。此外，由于在 LED 光条件下产生更高的 NADPH 含量，因此在蓝-红 LED 光条件下，苯酚、儿茶酚和顺式，顺式粘酸的生成量高于在白色荧光光条件下。因此，在蓝-红 LED 光条件下，绿宝石具有在污染场地中高效去除苯的潜力。
