Technical University of Berlin, Straße des 17. Juni 124, Berlin 10623, Germany.
UEKAE - BILGEM - The Scientific and Technological Research Council of Turkey (TUBITAK), Kocaeli 41470, Turkey.
Biosens Bioelectron. 2018 Feb 15;100:541-548. doi: 10.1016/j.bios.2017.09.046. Epub 2017 Sep 28.
A fully automated microfluidic-based electrochemical biosensor was designed and manufactured for pathogen detection. The quantification of Escherichia coli was investigated with standard and nanomaterial amplified immunoassays in the concentration ranges of 0.99 × 103.98 × 10 cfu mL and 103.97 × 10 cfu mL which resulted in detection limits of 1.99 × 10 cfu mL and 50 cfu mL, respectively. The developed methodology was then applied for E. coli quantification in water samples using nanomaterial modified assay. Same detection limit for E. coli was achieved for real sample analysis with a little decrease on the sensor signal. Cross-reactivity studies were conducted by testing Shigella, Salmonella spp., Salmonella typhimurium and Staphylococcus aureus on E. coli specific antibody surface that confirmed the high specificity of the developed immunoassays. The sensor surface could be regenerated multiple times which significantly reduces the cost of the system. Our custom-designed biosensor is capable of detecting bacteria with high sensitivity and specificity, and can serve as a promising tool for pathogen detection.
设计并制造了一种完全自动化的基于微流控的电化学生物传感器，用于病原体检测。使用标准和纳米材料放大免疫分析研究了大肠杆菌的定量检测，在 0.99×10^3.98×10^cfu mL 和 103.97×10^cfu mL 的浓度范围内，检测限分别为 1.99×10^cfu mL 和 50 cfu mL。然后，使用纳米材料修饰的测定法将开发的方法应用于水样中的大肠杆菌定量检测。对于实际样品分析，同样的检测限可以通过传感器信号略有下降来实现。通过在大肠杆菌特异性抗体表面上测试志贺氏菌、沙门氏菌属、鼠伤寒沙门氏菌和金黄色葡萄球菌，进行了交叉反应性研究，证实了所开发的免疫分析具有很高的特异性。传感器表面可以多次再生，这大大降低了系统的成本。我们定制设计的生物传感器能够以高灵敏度和特异性检测细菌，可作为病原体检测的有前途的工具。
