Okotrub K A, Amstislavsky S Y, Surovtsev N V
Institute of Automation and Electrometry, Russian Academy of Sciences, Novosibirsk 630090, Russia.
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk 630090, Russia; Novosibirsk State University, Novosibirsk, 630090, Russia.
Arch Biochem Biophys. 2017 Dec 1;635:37-43. doi: 10.1016/j.abb.2017.10.001. Epub 2017 Oct 6.
Although lipid phase transition is believed to be among the major damaging factors in oocytes and preimplantation embryos cryopreservation, lack of the appropriate experimental methods limits investigation of this phenomenon. Herein, we demonstrate the capabilities of Raman spectroscopy to detect the lipid phase transition within the freezing preimplantation mouse embryos. We exploit the sensibility of antisymmetric CH Raman peak to the phase state of lipids. It is shown that during the freezing of the mouse embryos the lipid phase transition occurs at the temperatures between -7 and 0 °C. Similar temperature dependences of CH mode intensities are found for lipids in the preimplantation embryos and a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, implying the similarity in the occupation rules of conformational states. Raman spectroscopy is considered as a method of choice to study the lipid phase transition during preimplantation mammalian embryos freezing and cryopreservation.
尽管脂质相变被认为是卵母细胞和植入前胚胎冷冻保存过程中的主要损伤因素之一，但缺乏合适的实验方法限制了对这一现象的研究。在此，我们展示了拉曼光谱在检测冷冻的植入前小鼠胚胎内脂质相变方面的能力。我们利用不对称CH拉曼峰对脂质相态的敏感性。结果表明，在小鼠胚胎冷冻过程中，脂质相变发生在-7至0°C之间的温度。在植入前胚胎中的脂质和1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱中发现了类似的CH模式强度温度依赖性，这意味着构象状态的占据规则具有相似性。拉曼光谱被认为是研究植入前哺乳动物胚胎冷冻和冷冻保存过程中脂质相变的首选方法。
