通过 3D 微流控芯片设计实现增强的表面声波细胞分选。

Enhanced surface acoustic wave cell sorting by 3D microfluidic-chip design.

机构信息

School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

出版信息

Lab Chip. 2017 Nov 21;17(23):4059-4069. doi: 10.1039/c7lc00715a.

Abstract

We demonstrate an acoustic wave driven microfluidic cell sorter that combines advantages of multilayer device fabrication with planar surface acoustic wave excitation. We harness the strong vertical component of the refracted acoustic wave to enhance cell actuation by using an asymmetric flow field to increase cell deflection. Precise control of the 3-dimensional flow is realized by topographical structures implemented on the top of the microchannel. We experimentally quantify the effect of the structure dimensions and acoustic parameter. The design attains cell sorting rates and purities approaching those of state of the art fluorescence-activated cell sorters with all the advantages of microfluidic cell sorting.

摘要

我们展示了一种基于声波驱动的微流控细胞分选器，它结合了多层器件制造和平面声波激励的优点。我们利用折射声波的强垂直分量，通过使用非对称流场来增加细胞偏转，从而增强细胞的驱动。通过在微通道顶部实现的形貌结构来实现对三维流场的精确控制。我们实验量化了结构尺寸和声学参数的影响。该设计实现了接近基于荧光激活细胞分选器的细胞分选速度和纯度，同时具有微流控细胞分选的所有优势。