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[甘草标准汤剂的研究]

[Research on Glycyrrhizeae Radix standard decoction].

作者信息

Lin Wei-Xiong, Le Zhi-Yong, Che Hai-Yan, Liu Yin-Zhen, Liu Chong, Wang Hui

机构信息

Guangdong Pharmaceutical University, Guangzhou 510006, China.

Kangmei Pharmaceutical Research Institute Co., Ltd., Beijing 102629, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2017 Mar;42(5):830-835. doi: 10.19540/j.cnki.cjcmm.20161222.070.

Abstract

Fifteen batches of Glycyrrhizeae Radix standard decoction were prepared for determination of the content of the glycyrrhizic acid and liquiritin, then the transfer rate and the extract rate were calculated and a method was established to analyze the fingerprint by HPLC. According to the measurement of 15 batches of samples,the transfer rate of the glycyrrhizic acid and liquiritin were 59.4%-87.4% and 49.8%-78.9% with extract rate of 29.9%-38.9%. Moreover,10 common chromatographic peaks were determined based on their fingerprint by using similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (TCM)(2012A) .The similarity results of 15 batches of samples were analyzed and compared,and the results showed that the similarity was all higher than 0.9. Fifteen batches of samples,prepared by a standard method,have stable quality and the high similarity.The method displayed good precision,stability and repeatability in fingerprint analysis. Therefore,this study can provide a reference for the quality control of Glycyrrhizae Radix dispensing granules.

摘要

制备15批甘草标准汤剂,测定甘草酸和甘草苷的含量,计算转移率和提取率,并建立高效液相色谱法分析指纹图谱的方法。根据15批样品的测定结果,甘草酸和甘草苷的转移率分别为59.4%-87.4%和49.8%-78.9%,提取率为29.9%-38.9%。此外,采用《中药色谱指纹图谱相似度评价系统》(2012版),基于指纹图谱确定了10个共有色谱峰。对15批样品的相似度结果进行分析比较,结果表明相似度均高于0.9。15批按标准方法制备的样品质量稳定,相似度高。该方法在指纹图谱分析中显示出良好的精密度、稳定性和重复性。因此,本研究可为甘草配方颗粒的质量控制提供参考。

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