Yang Pei-Lei, Jiang Jian-Ping, Li Quan-Qing, Wu Xu-Ming, Chen Zhi-Yun
Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
The First Affiliated Hospital of Zhejiang Chinese Medical University, Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310006, China.
Zhongguo Zhong Yao Za Zhi. 2017 Mar;42(5):936-943. doi: 10.19540/j.cnki.cjcmm.20170121.008.
To observe and investigate the effects and mechanisms of the pure total flavonoids from Citrus changshan-huyou(PTFC) on blood lipid metabolism in hyperlipidemic rats. SD rats were fed with high fat diet for 4 weeks to induce hyperlipidemic rats model, meanwhile three dosages (50, 100, 200 mg•kg ⁻¹•d ⁻¹) of PTFC were administrated intragastrically for 4 weeks respectively.After 2 weeks of modeling, their tail blood was taken and serum TC, TG, and HDL-C levels were detected by biochemical method and their body weight was measured. After 4 weeks of modeling, their body weight was measured and liver weight was measured, then the levels of TC, TG, HDL-C, LDL-C, ALT, AST, MDA and SOD in serum were detected to calculate lipid comprehensive index(LDL-C/HDL-C and LDL-C/TC ratios) and atherogenic index(AI); in addition, MDA and SOD levels were detected by biochemical method. The hitopathological changes of the liver tissues were observed by HE staining; the protein expression levels of PPAR-α, Lpl, and Lipc were detected by ELISA; and the mRNA expression levels of PPAR-α in the liver tissue were detected by Real-time PCR. The results showed that gavage administration of the PTFC significantly decreased the body weight, liver weight, liver index, serum ALT and AST activities, the levels of serum TC, TG, LDL-C, LDL-C/HDL-C, AI and increased serum HDL and LDL/TC level. Moreover, the PTFC significantly enhanced SOD activity and decreased the concentration of MDA in serum and liver tissue. Further mechanism investigation indicated that PTFC inhibited serum lipid accumulation by increasing the expressions PPAR-α, Lpl, Lipc protein and PPAR-α mRNA of the liver tissues. PTFC could actively regulate blood lipid metabolism by ameliorating hepatic function, improving the body's antioxidant capacity, lowering levels of oxidative stress, as well as positively regulating the expression levels of PPAR-α, Lpl, Lipc protein and PPAR-α mRNA of the liver tissues in rats.
观察并研究常山胡柚纯总黄酮（PTFC）对高脂血症大鼠血脂代谢的影响及其机制。将SD大鼠喂饲高脂饲料4周以诱导高脂血症大鼠模型，同时分别以三种剂量（50、100、200mg•kg⁻¹•d⁻¹）的PTFC灌胃给药4周。造模2周后，采集其尾血，采用生化方法检测血清总胆固醇（TC）、甘油三酯（TG）和高密度脂蛋白胆固醇（HDL-C）水平，并测量体重。造模4周后，测量体重和肝脏重量，然后检测血清中TC、TG、HDL-C、低密度脂蛋白胆固醇（LDL-C）、谷丙转氨酶（ALT）、谷草转氨酶（AST）、丙二醛（MDA）和超氧化物歧化酶（SOD）水平，计算血脂综合指数（LDL-C/HDL-C和LDL-C/TC比值）和动脉粥样硬化指数（AI）；此外，采用生化方法检测MDA和SOD水平。通过苏木精-伊红（HE）染色观察肝组织的病理变化；采用酶联免疫吸附测定（ELISA）法检测过氧化物酶体增殖物激活受体α（PPAR-α）、脂蛋白脂肪酶（Lpl）和肝脂酶（Lipc）的蛋白表达水平；采用实时荧光定量聚合酶链反应（Real-time PCR）法检测肝组织中PPAR-α的mRNA表达水平。结果表明，灌胃给予PTFC可显著降低体重、肝脏重量、肝脏指数、血清ALT和AST活性、血清TC、TG、LDL-C、LDL-C/HDL-C、AI水平，并升高血清HDL和LDL/TC水平。此外，PTFC可显著增强血清和肝组织中SOD活性，降低MDA浓度。进一步机制研究表明，PTFC通过增加肝组织中PPAR-α、Lpl、Lipc蛋白和PPAR-α mRNA的表达来抑制血清脂质蓄积。PTFC可通过改善肝功能、提高机体抗氧化能力、降低氧化应激水平以及正向调节大鼠肝组织中PPAR-α、Lpl、Lipc蛋白和PPAR-α mRNA的表达水平来积极调节血脂代谢。
