Pastuszko A, Wilson D F
Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Cancer Biochem Biophys. 1988 May;9(4):359-66.
Four different amino acids (kainate, N-methyl-D-aspartate, L-cysteine sulfinate and D,L-2-amino-5-phosphonovalerate) have been observed to stimulate uptake of 45Ca2+ into human neuroblastoma cells. This stimulation of uptake is specific and many amino acids which are structural analogs of the above compounds are without activity. The calcium movement is not inhibited by compounds which block voltage-dependent calcium channels. Biological specificity is observed in which some cell lines respond to the amino acids and others do not. It is concluded that these amino acids are acting on a class of receptors whose physiological role is modulation of neuronal metabolism by modulating the calcium permeability of the plasma membrane. The amino acids can substitute for the, as yet, unidentified natural agonists, albeit with low affinity.
已观察到四种不同的氨基酸（海人酸、N-甲基-D-天冬氨酸、L-半胱氨酸亚磺酸盐和D,L-2-氨基-5-膦酸戊酸）能刺激45Ca2+摄入人神经母细胞瘤细胞。这种摄取刺激具有特异性，上述化合物的许多结构类似物氨基酸没有活性。钙的移动不受阻断电压依赖性钙通道的化合物抑制。观察到生物特异性，即一些细胞系对这些氨基酸有反应，而另一些则没有。得出的结论是，这些氨基酸作用于一类受体，其生理作用是通过调节质膜的钙通透性来调节神经元代谢。这些氨基酸可以替代尚未确定的天然激动剂，尽管亲和力较低。
