Yamaguchi M, Imase M
Department of Environmental Biochemistry, Shizuoka College of Pharmacy, Japan.
Horm Metab Res. 1988 Apr;20(4):221-4. doi: 10.1055/s-2007-1010798.
The effect of synthetic [Asu1,7] eel calcitonin (CT) and other hormones on biliary calcium excretion was investigated in rats cannulated bile duct. Administration of CT (80 mU/100 g body weight) produced a significant increase in liver calcium and a corresponding elevation of bile calcium content. The increase in bile calcium content was also caused by administration of insulin (0.1 U/100 g), epidermal growth factor (10 micrograms/100 g), glucagon (10 micrograms/100 g), epinephrine (10 micrograms/100 g), norepinephrine (10 micrograms/100 g), 4 beta-phorbol 12-myristate-13-acetate (10 micrograms/100 g) and ATP (1.0 mg/100 g), suggesting that this increase may be a receptors-mediated response. Of these hormones and drugs, norepinephrine, a alpha-receptor mediator, clearly prevented CT effect on biliary calcium excretion. Moreover, phenylephrine, a alpha 1-receptor agonist, caused an inhibition of the CT effect, while the agonist significantly increased biliary calcium excretion. The present study clearly demonstrates that biliary calcium excretion is stimulated by various hormones which increase calcium influx into liver cells, and suggests that the CT action may be inhibited by alpha 1-adrenergic stimulation.
在大鼠胆管插管模型中研究了合成的[Asu1,7]鳗鱼降钙素（CT）及其他激素对胆汁钙排泄的影响。给予CT（80 mU/100 g体重）可使肝脏钙含量显著增加，胆汁钙含量相应升高。给予胰岛素（0.1 U/100 g）、表皮生长因子（10微克/100 g）、胰高血糖素（10微克/100 g）、肾上腺素（10微克/100 g）、去甲肾上腺素（10微克/100 g）、4β-佛波醇12-肉豆蔻酸酯-13-乙酸酯（10微克/100 g）和ATP（1.0 mg/100 g）也可导致胆汁钙含量增加，提示这种增加可能是受体介导的反应。在这些激素和药物中，α受体介质去甲肾上腺素明显抑制CT对胆汁钙排泄的作用。此外，α1受体激动剂去氧肾上腺素可抑制CT的作用，而该激动剂可显著增加胆汁钙排泄。本研究清楚地表明，多种激素可刺激胆汁钙排泄，这些激素增加了钙流入肝细胞的量，并提示α1肾上腺素能刺激可能抑制CT的作用。
