兔植入前胚泡和黄体体外从胆固醇生成雌激素的过程。

Estrogen formation from cholesterol in rabbit preimplantation blastocysts and corpora lutea in vitro.

作者信息

Tanaka T, Fujimoto S, Sakuragi N, Ichinoe K

机构信息

Department of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Japan.

出版信息

Int J Fertil. 1988 May-Jun;33(3):212-5.

PMID:2899569

Abstract

Rabbit blastocysts and corpora lutea (CL) obtained six days after coitus were incubated with [4-14C]cholesterol. In the CL, the conversion of [4-14C]cholesterol to radioactive progesterone was about twice that to radioactive estrone. On the other hand, in the preimplantation blastocyst, estrone formation from the substrate was far higher than progesterone formation. Both in the blastocyst and CL, [4-14C]cholesterol was least incorporated into estradiol among the steroids identified. It is suggested that steroids biosynthesized in rabbit preimplantation blastocysts and CL are quite different both in quality and in quantity at the time of implantation.

摘要

将交配后6天获得的兔胚泡和黄体（CL）与[4-¹⁴C]胆固醇一起孵育。在黄体中，[4-¹⁴C]胆固醇转化为放射性孕酮的量约为转化为放射性雌酮量的两倍。另一方面，在植入前的胚泡中，底物形成雌酮的量远高于形成孕酮的量。在胚泡和黄体中，在所鉴定的类固醇中，[4-¹⁴C]胆固醇掺入雌二醇的量最少。这表明在植入时，兔植入前胚泡和黄体中生物合成的类固醇在质量和数量上都有很大差异。