Bhattacharya-Chatterjee M, Chatterjee S K, Vasile S, Seon B K, Kohler H
Department of Gynecologic Oncology, Roswell Park Memorial Institute, Buffalo, NY 14263.
J Immunol. 1988 Aug 15;141(4):1398-403.
Previously, we had generated anti-Id mAb (Ab2) binding to a hybridoma SN2 (Ab1), which recognizes a glycoprotein, gp37, expressed by human leukemic T cells. To characterize these anti-idiotopes further, they were used to immunize mice and rabbits. Several murine anti-anti-Idiotype mAb (Ab3), mostly of IgM-k isotype, were obtained. mAb3 and sera from rabbits immunized with Ab2 contained antibodies that bind to gp37 Ag and leukemic MOLT-4 and JM cells. Also, mAb3 and immune sera from rabbits competed with Ab1 for binding to MOLT-4 cells. They inhibited the binding of iodinated Ab1 to Ab2 indicating that Ab3 in mice and rabbits shares idiotopes with Ab1 (SN2). Furthermore, both the murine mAb3 and rabbit polyclonal Ab3 immunoprecipitated the same gp37 Ag as SN2 (Ab1). The production of Ag-specific Ab3 (Ab1') in mice and rabbits in absence of any exposure to gp37 indicates that these Ab2 may indeed carry the internal image of the gp37 Ag. Such anti-idiotopes (Ab2 beta) may be useful as Ag substitute for the induction of therapeutic immunity in T cell leukemia patients.
此前，我们制备了与杂交瘤SN2（Ab1）结合的抗独特型单克隆抗体（Ab2），该杂交瘤识别一种由人白血病T细胞表达的糖蛋白gp37。为了进一步表征这些抗独特型，用它们免疫小鼠和兔子。获得了几种鼠抗抗独特型单克隆抗体（Ab3），大多为IgM-k同种型。用Ab2免疫的兔子的mAb3和血清含有能与gp37抗原、白血病MOLT-4和JM细胞结合的抗体。此外，来自兔子的mAb3和免疫血清与Ab1竞争与MOLT-4细胞的结合。它们抑制了碘化Ab1与Ab2的结合，表明小鼠和兔子中的Ab3与Ab1（SN2）共享独特型。此外，鼠mAb3和兔多克隆Ab3都免疫沉淀了与SN2（Ab1）相同的gp37抗原。在未接触任何gp37的情况下，小鼠和兔子中产生抗原特异性Ab3（Ab1'）表明这些Ab2可能确实携带gp37抗原的内影像。这种抗独特型（Ab2β）可用作抗原替代物，用于诱导T细胞白血病患者的治疗性免疫。
