Amann E, Abel K J, Grundmann U, Okazaki H, Küpper H A
Research Laboratories of Behringwerke AG, Marburg, W. Germany.
Behring Inst Mitt. 1988 Apr(82):35-42.
The coding sequence for human factor XIIIa (FXIIIa) was introduced into Escherichia coli expression vectors. Bacterial cells transformed with the recombinant plasmids synthesized fusion proteins of the expected molecular weights and the proteins were shown to be immunoreactive with anti-FXIII antibodies. Furthermore, with the help of oligodeoxynucleotide synthesis, we constructed a plasmid which directs the synthesis of the human FXIIIa protein in the unfused form. Sequence determination at the aminoterminus of this protein revealed the identical sequence compared to placental FXIIIa. The protein is expressed intracellularly in a denatured and biologically inactive form. It constitutes approximately 2% of total cellular protein and can easily be purified by standard methods.
将人凝血因子 XIIIa（FXIIIa）的编码序列导入大肠杆菌表达载体。用重组质粒转化的细菌细胞合成了预期分子量的融合蛋白，并且这些蛋白显示与抗 FXIII 抗体具有免疫反应性。此外，借助寡脱氧核苷酸合成，我们构建了一个质粒，该质粒指导未融合形式的人 FXIIIa 蛋白的合成。对该蛋白氨基末端的序列测定显示，其与胎盘 FXIIIa 的序列相同。该蛋白以变性且无生物学活性的形式在细胞内表达。它约占细胞总蛋白的 2%，并且可以通过标准方法轻松纯化。
