Furukawa K, Mochizuki Y, Sawada N, Gotoh M, Tsukada H
Department of Pathology, Sapporo Medical College, Japan.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;55(5):279-85. doi: 10.1007/BF02896586.
The present ultrastructural morphometric and cytochemical studies demonstrate clofibrate induced changes in peroxisomes in adult rat hepatocytes maintained for 14 days in primary culture on floating collagen gels. Catalase activity and the number and diameter of peroxisomes were reduced in hepatocytes cultured for between 2/3 and 7 days. However, hepatocytes cultured for 7-14 days had well-developed peroxisomes containing crystalloid nucleoids. The number of anucleoid peroxisomes in hepatocytes treated with 2 mM Na clofibrate increased with culture age, and by day 14 the number was 2.9 times greater than in freshly isolated hepatocytes. Catalase activity, as well as the number of nucleoid-containing peroxisomes were much greater in treated hepatocytes than in untreated controls, but decreased slightly with culture age. The diameter of peroxisomes was not reduced in the treated cells. These results suggest that the treatment with Na clofibrate is effective both for proliferation and maintenance of peroxisomes and for enhancing catalase activity. In treated hepatocytes, matrical plates were formed in peroxisomes from days 5 to 14 and the number of plate-containing peroxisomes increased with culture age.
目前的超微结构形态计量学和细胞化学研究表明，在漂浮胶原凝胶上原代培养14天的成年大鼠肝细胞中，氯贝丁酯可诱导过氧化物酶体发生变化。培养2/3至7天的肝细胞中，过氧化氢酶活性以及过氧化物酶体的数量和直径均降低。然而，培养7 - 14天的肝细胞具有发育良好的含有类晶体核仁的过氧化物酶体。用2 mM氯贝丁酸钠处理的肝细胞中无核仁过氧化物酶体的数量随培养时间增加，到第14天时，其数量比新鲜分离的肝细胞多2.9倍。处理过的肝细胞中过氧化氢酶活性以及含核仁过氧化物酶体的数量均比未处理的对照细胞多，但随培养时间略有下降。处理过的细胞中过氧化物酶体的直径未减小。这些结果表明，氯贝丁酸钠处理对于过氧化物酶体的增殖、维持以及增强过氧化氢酶活性均有效。在处理过的肝细胞中，从第5天到第14天过氧化物酶体中形成了基质板，且含板过氧化物酶体的数量随培养时间增加。
