Brouwers H A, Overbeeke M A, van Ertbruggen I, Schaasberg W, Alsbach G P, van der Heiden C, van Leeuwen E F, Stoop J W, Engelfriet C P
University Hospital for Children and Youth Het Wilhelmina Kinderziekenhuis, Utrecht, The Netherlands.
Lancet. 1988 Sep 17;2(8612):641-4. doi: 10.1016/s0140-6736(88)90466-7.
In 80 newborn infants ABO-incompatible with their mothers, the lysis-inducing effect of the maternal IgG anti-A or anti-B antibodies in an antibody-dependent cell-mediated cytotoxicity (ADCC) assay and the antigen density of A or B antigens on the red cells of the children were measured. On the basis of the results, the children were divided into two groups--24 children in whom increased haemolysis was to be expected, and 56 children in whom it was not. Signs of haemolysis and serological features of ABO haemolytic disease of the newborn (ABO-HDN) were compared in these two groups and a control group of 120 ABO-compatible infants. The effect of the maternal antibodies in the ADCC assay, the titres of maternal IgG anti-A or anti-B antibodies, the results of the direct antiglobulin test on the red cells in the cord blood, and the titre of IgG anti-A or anti-B antibodies in the serum of the infants were compared for their ability to predict the severity of ABO-HDN. This was also done for the combination of the ADCC assay results plus the A or B antigen density and the direct antiglobulin test plus the titre of maternal IgG anti-A or anti-B antibodies. The ADCC assay with maternal serum was the most sensitive assay to predict ABO-HDN, and the combination of the ADCC assay with A or B antigen density determination the most specific test.
在80例与母亲ABO血型不相容的新生儿中，检测了母体IgG抗A或抗B抗体在抗体依赖细胞介导的细胞毒性（ADCC）试验中的溶血诱导作用以及患儿红细胞上A或B抗原的抗原密度。根据检测结果，将患儿分为两组——预计溶血会增加的24例患儿和预计不会增加的56例患儿。比较了这两组以及120例ABO血型相容婴儿的对照组中新生儿ABO溶血病（ABO-HDN）的溶血迹象和血清学特征。比较了ADCC试验中母体抗体的作用、母体IgG抗A或抗B抗体的滴度、脐血红细胞直接抗球蛋白试验的结果以及婴儿血清中IgG抗A或抗B抗体的滴度预测ABO-HDN严重程度的能力。对于ADCC试验结果加A或B抗原密度以及直接抗球蛋白试验加母体IgG抗A或抗B抗体滴度的组合，也进行了上述比较。用母体血清进行的ADCC试验是预测ABO-HDN最敏感的试验，而ADCC试验与A或B抗原密度测定相结合是最具特异性的检测方法。
