Perez Victor L
*the Department of Ophthalmology, Bascom Palmer Eye Institute; and †the Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL.
Cornea. 2017 Nov;36 Suppl 1:S5-S8. doi: 10.1097/ICO.0000000000001354.
The eye has become a useful site for the investigation and understanding of local and systemic immune responses. The ease of access and transparency of the cornea permits direct visualization of ocular structures, blood vessels, and lymphatic vessels, allowing for the tracking of normal and pathological biological processes in real time. As a window to the immune system, we have used the eye to dissect the mechanisms of corneal inflammatory reactions that include innate and adaptive immune responses. We have identified that the ocular microenvironment regulates these immune responses by recruiting different populations of inflammatory cells to the cornea through local production of selected chemokines. Moreover, crosstalk between T cells and macrophages is a common and crucial step in the development of ocular immune responses to corneal alloantigens. This review summarizes the data generated by our group using intravital fluorescent confocal microscopy to capture the tempo, magnitude, and function of innate and adaptive corneal immune responses.
眼睛已成为研究和理解局部及全身免疫反应的有用部位。角膜易于接近且具有透明度，这使得能够直接观察眼部结构、血管和淋巴管，从而实时追踪正常和病理生物学过程。作为免疫系统的一扇窗口，我们利用眼睛剖析了角膜炎症反应的机制，其中包括先天性和适应性免疫反应。我们已经确定，眼部微环境通过局部产生特定趋化因子将不同群体的炎性细胞募集到角膜，从而调节这些免疫反应。此外，T细胞与巨噬细胞之间的相互作用是眼部对角膜同种异体抗原免疫反应发展过程中一个常见且关键的步骤。本综述总结了我们团队通过活体荧光共聚焦显微镜生成的数据，以捕捉先天性和适应性角膜免疫反应的节奏、强度和功能。
