The ionic mechanisms underlying opioid actions.

Recent experiments using intracellular recording techniques in vitro have revealed that common ionic mechanisms may explain the actions of opioid drugs. Evidence is now available from studies on guinea pig gut myenteric and submucous plexi, from preparations of spinal cord and dorsal root ganglia, from brain slices including the locus coeruleus and from neuroblastoma/glioma hybrid cells. The concensus is that mu opioid receptors activate an outward potassium conductance, possibly by way of adenylate cyclase. Activation of the receptor increases the membrane permeability to potassium ions and thus produces a membrane hyperpolarisation and conductance increase, plus an indirect inhibition of calcium entry during the action potential. Kappa opioids appear to inhibit directly the entry of calcium through voltage-dependent calcium channels, although to date there is no conclusive evidence that this mechanism of action can be extended to neurones of the central nervous system. The mechanism of action of delta opioids has only recently been investigated and initial evidence suggests they increase a potassium conductance similar to that increased by mu opioids. However, work in neuroblastoma x glioma hybrid cells has suggested that in these cells at least, receptor activation depress a component of voltage-dependent calcium current. The link between the receptor and the calcium channel involves a G-protein, Go.