Sol Yuri, Choi Seon Hee, Kim Hak Jin, Kim Yong-Woo, Cho Byung Mann, Han Hyung Soo, Choi Ki J
Department of Radiology, School of Medicine, Pusan National University, Biomedical Research Institute, Pusan National University Hospital, Pusan 179, Gudeokro, Seogu 602-739, Busan.
Department of Preventive Medicine, School of Medicine, Pusan National University, Yangsan.
Microscopy (Oxf). 2017 Oct 1;66(5):366-370. doi: 10.1093/jmicro/dfx026.
Triolein emulsion has been known to increase vascular permeability in the brain when it is infused into the carotid artery. The purpose of this study was to identify the morphologic mechanism of increased vascular permeability in brain induced by infusion of emulsified triolein into the carotid artery by transmission electron microscopy (TEM). Triolein emulsion was infused into the carotid artery of rats. TEM using lanthanum tracer was used to evaluate morphologic changes in endothelium with a focus on transcytotic vesicles and tight junction opening. The treat group showed multiple transcytotic vesicles containing lanthanum tracer within endothelium on TEM. TEM also revealed that lanthanum tracer entered neural interstitium through tight junctions between capillary endothelial cells infrequently in the treat group. No evidence of transcytotic vesicles containing lanthanum tracer or lanthanum leakage through tight junctions was observed in the control group. Transcytosis and the opening of tight junctions appears the pathway for vascular permeability enhancement by triolein. This result could be utilized in studies on the blood-brain barrier and by those searching for chemotherapeutic methods that deliver anti-tumor agents to normally drug inaccessible organs.
已知将三油酸甘油酯乳剂注入颈动脉时会增加脑内血管通透性。本研究的目的是通过透射电子显微镜（TEM）确定经颈动脉注入乳化三油酸甘油酯所致脑内血管通透性增加的形态学机制。将三油酸甘油酯乳剂注入大鼠颈动脉。使用镧示踪剂的TEM用于评估内皮细胞的形态学变化，重点是穿胞小泡和紧密连接开放情况。治疗组在TEM下可见内皮细胞内有多个含有镧示踪剂的穿胞小泡。TEM还显示，治疗组中镧示踪剂很少通过毛细血管内皮细胞之间的紧密连接进入神经间质。对照组未观察到含有镧示踪剂的穿胞小泡或镧通过紧密连接泄漏的证据。穿胞作用和紧密连接开放似乎是三油酸甘油酯增强血管通透性的途径。这一结果可用于血脑屏障研究以及那些寻找将抗肿瘤药物输送到正常情况下药物难以到达的器官的化疗方法的研究中。
