Guy J, Pelletier G
MRC Group in Molecular Endocrinology, Laval University Medical Center, Ste-Foy, Quebec, Canada.
Peptides. 1988 May-Jun;9(3):567-70. doi: 10.1016/0196-9781(88)90165-9.
Several recent studies have suggested interactions between catecholamine (CA) and neuropeptide Y (NPY) neuronal systems in the rat brain. In order to obtain morphological evidence for such CA/NPY interactions in the arcuate nucleus, we have used a double immunostaining procedure using an anti-tyrosine hydroxylase (TH) antiserum as a marker for catecholamine neurons and an anti-NPY antiserum. This double staining, where the first staining is silver-gold intensified, was detectable at both light and electron microscopic levels. In semi-thin sections, a substantial overlap and close proximity of TH-immunopositive neurons and NPY neuronal elements could be seen within the arcuate nucleus. At the electron microscopic level, direct appositions between TH- and NPY-immunoreactive structures could be detected. These appositions were of axosomatic, axodendritic or axoaxonic types without any synaptic membrane differentiation. Moreover, direct appositions between NPY-immunoreactive structures have also been observed. This morphological study showing appositions between TH and NPY neuronal systems suggest direct interactions between these two systems in the arcuate nucleus.
最近的几项研究表明，大鼠脑中儿茶酚胺（CA）和神经肽Y（NPY）神经元系统之间存在相互作用。为了获得弓状核中这种CA/NPY相互作用的形态学证据，我们使用了双重免疫染色程序，用抗酪氨酸羟化酶（TH）抗血清作为儿茶酚胺神经元的标志物，并用抗NPY抗血清。这种双重染色，其中第一次染色用银金强化，在光学和电子显微镜水平上均可检测到。在半薄切片中，可以看到弓状核内TH免疫阳性神经元和NPY神经元成分有大量重叠且紧密相邻。在电子显微镜水平上，可以检测到TH和NPY免疫反应性结构之间的直接并置。这些并置为轴体、轴树突或轴轴突类型，没有任何突触膜分化。此外，还观察到NPY免疫反应性结构之间的直接并置。这项显示TH和NPY神经元系统之间并置的形态学研究表明，这两个系统在弓状核中存在直接相互作用。
