Foito Alexandre, Hackett Christine Anne, Stewart Derek, Velmurugan Janaki, Milbourne Dan, Byrne Stephen L, Barth Susanne
Teagasc, Crops Environment and Land Use Programme, Oak Park Research Centre, Carlow, Ireland.
Enhancing Crop Productivity and Utilisation, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
BMC Genet. 2017 Oct 10;18(1):84. doi: 10.1186/s12863-017-0552-0.
Recent advances in the mapping of biochemical traits have been reported in Lolium perenne. Although the mapped traits, including individual sugars and fatty acids, contribute greatly towards ruminant productivity, organic acids and amino acids have been largely understudied despite their influence on the ruminal microbiome.
In this study, we used a targeted gas-chromatography mass spectrometry (GC-MS) approach to profile the levels of 25 polar metabolites from different classes (sugars, amino acids, phenolic acids, organic acids and other nitrogen-containing compounds) present in a L. perenne F2 population consisting of 325 individuals. A quantitative trait (QTL) mapping approach was applied and successfully identified QTLs regulating seven of those polar metabolites (L-serine, L-leucine, glucose, fructose, myo-inositol, citric acid and 2, 3-hydroxypropanoic acid).Two QTL mapping approaches were carried out using SNP markers on about half of the population only and an imputation approach using SNP and DArT markers on the entire population. The imputation approach confirmed the four QTLs found in the SNP-only analysis and identified a further seven QTLs.
These results highlight the potential of utilising molecular assisted breeding in perennial ryegrass to modulate a range of biochemical quality traits with downstream effects in livestock productivity and ruminal digestion.
黑麦草在生化特性图谱绘制方面已有近期进展报道。尽管已绘制的特性，包括单糖和脂肪酸，对反刍动物生产力有很大贡献，但有机酸和氨基酸尽管对瘤胃微生物群有影响，却在很大程度上未被充分研究。
在本研究中，我们采用靶向气相色谱 - 质谱联用（GC-MS）方法，对由325个个体组成的黑麦草F2群体中存在的不同类别（糖、氨基酸、酚酸、有机酸和其他含氮化合物）的25种极性代谢物水平进行了分析。应用了数量性状（QTL）定位方法，并成功鉴定出调控其中7种极性代谢物（L-丝氨酸、L-亮氨酸、葡萄糖、果糖、肌醇、柠檬酸和2,3-羟基丙酸）的QTL。使用SNP标记仅对大约一半的群体进行了两种QTL定位方法，并对整个群体使用SNP和DArT标记进行了填充方法。填充方法证实了仅SNP分析中发现的4个QTL，并鉴定出另外7个QTL。
这些结果突出了利用多年生黑麦草分子辅助育种来调节一系列生化品质性状的潜力，这些性状对家畜生产力和瘤胃消化具有下游影响。
