Eriksdotter-Nilsson M, Meister B, Hökfelt T, Elde R, Fahrenkrug J, Frey P, Oertel W, Rehfeld J F, Terenius L, Olson L
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
Synapse. 1987;1(6):539-51. doi: 10.1002/syn.890010606.
Fetal parietal cerebral cortex was transplanted to the anterior eye chamber of adult Sprague-Dawley rats. After two to three months the grafts, with or without colchicine treatment, were subjected to immunohistochemical analysis using antibodies against cholecystokinin (CCK), somatostatin (SOM), neuropeptide tyrosine (NPY), vasoactive intestinal polypeptide (VIP), peptide histidine isoleucine (PHI) and the GABA-synthesizing enzyme glutamic acid decarboxylase (GAD). Cerebral cortex in situ of untreated and colchicine-treated rats was always analyzed in parallel. A dense plexus of CCK-immunoreactive fibers was distributed in all parts of the transplants, and after colchicine treatment a large number of CCK-positive cells was observed. These cells were markedly increased in number as compared to normal cortical tissue in colchicine-pretreated rats. The amount of NPY-immunoreactive cells was also markedly increased, whereas somatostatin-positive cells were found in numbers similar to those seen in cortex in situ. In the grafts only a few VIP- and PHI-positive fibers were seen with a few VIP-positive cell bodies, but no clearly discernible PHI-positive cells. A very dense plexus of GAD-positive fibers with an even distribution throughout the grafts was observed. Cortex in situ exhibited a lower density of GAD-immunoreactive fibers. Even after colchicine treatment the number of GAD-positive cells in the grafts was low. Using double-staining techniques, it was found that most of the few GAD-positive cells in the grafts were also NPY-positive, SOM-positive or, to a minor extent, CCK-positive. The present results demonstrate that several peptides and transmitter markers are expressed in cortical grafts in oculo, but marked differences in their expression can be observed in cortical tissue that has developed in isolation. Thus, the intraocular cortex graft, alone and in combination with other brain areas, should provide a useful model in which to study factors that regulate brain development.
将胎鼠顶叶大脑皮质移植到成年斯普拉格 - 道利大鼠的前房。两到三个月后，对移植组织进行秋水仙碱处理或未处理，然后使用抗胆囊收缩素（CCK）、生长抑素（SOM）、神经肽Y（NPY）、血管活性肠肽（VIP）、肽组氨酸异亮氨酸（PHI）以及γ-氨基丁酸合成酶谷氨酸脱羧酶（GAD）的抗体进行免疫组织化学分析。同时，对未处理和经秋水仙碱处理的大鼠的原位大脑皮质进行平行分析。移植组织的各个部位均分布有密集的CCK免疫反应性纤维丛，秋水仙碱处理后观察到大量CCK阳性细胞。与秋水仙碱预处理大鼠的正常皮质组织相比，这些细胞的数量明显增加。NPY免疫反应性细胞的数量也显著增加，而生长抑素阳性细胞的数量与原位皮质中的数量相似。在移植组织中，仅可见少数VIP和PHI阳性纤维以及少数VIP阳性细胞体，但未见明显可辨别的PHI阳性细胞。观察到GAD阳性纤维丛非常密集，均匀分布于整个移植组织中。原位皮质中GAD免疫反应性纤维的密度较低。即使经过秋水仙碱处理，移植组织中GAD阳性细胞的数量仍然很少。采用双重染色技术发现，移植组织中少数GAD阳性细胞大多也为NPY阳性、SOM阳性或在较小程度上为CCK阳性。目前的结果表明，几种肽和递质标记物在眼内皮质移植组织中表达，但在孤立发育的皮质组织中可观察到它们表达的显著差异。因此眼内皮质移植组织单独或与其他脑区联合，应能为研究调节脑发育的因素提供一个有用的模型。
