Jagannath S, Reading C L, Dicke K A, Tindle S, Devaraj B, Tucker S L, Spitzer G
Department of Hematology, University of Texas M.D. Anderson Hospital, Houston 77030.
Bone Marrow Transplant. 1987 Feb;1(3):281-8.
We have evaluated the transplantation potential of bone marrow stem cell concentrates isolated from the 40/60% interface of discontinuous Percoll gradients. This mononuclear fraction is free from platelets and depleted of granulocytes, and contains the majority of granulocyte-macrophage colony-forming cells (GM-CFC), erythroid burst-forming units (BFU-E), and granulocyte, erythroid, macrophage, megakaryocyte colony-forming cells (GEMM-CFC) in less than 10% of the cell number of the original buffy coat. This preparation allows further manipulation without the clumping and cell loss associated with buffy coat cell preparations. Cells isolated by this technique were evaluated for hematopoietic restoration potential in 14 patients who received allogeneic bone marrow transplants as supportive therapy after high dose cytoreduction to treat leukemias or lymphoma. The number of nucleated cells infused varied from 1.6-5.5 X 10(7)/kg, and the number of GM-CFC infused ranged from 0.4 to 3.7 X 10(5)/kg. There was an inverse relationship between the time to recovery of granulocytes and platelets and the number of GM-CFC infused when fewer than 10(5) GM-CFC/kg were transplanted. Above this dose, there was recovery within 10-15 days after transplantation. The stem cell-enriched fraction contained 30-40% of the original number of T lymphocytes, and acute graft-versus-host disease was observed in seven of these patients.
我们评估了从不连续 Percoll 梯度的 40/60% 界面分离出的骨髓干细胞浓缩物的移植潜力。这个单核部分不含血小板且粒细胞减少，并且在原始血沉棕黄层细胞数量的不到 10% 中包含了大部分粒细胞 - 巨噬细胞集落形成细胞（GM-CFC）、红系爆式集落形成单位（BFU-E）以及粒细胞、红系、巨噬细胞、巨核细胞集落形成细胞（GEMM-CFC）。这种制剂允许进一步操作，而不会出现与血沉棕黄层细胞制剂相关的细胞聚集和细胞损失。通过该技术分离的细胞在 14 例接受异基因骨髓移植作为高剂量细胞减灭术后支持治疗以治疗白血病或淋巴瘤的患者中评估了造血恢复潜力。输注的有核细胞数量在 1.6 - 5.5×10⁷/kg 之间，输注的 GM-CFC 数量在 0.4 至 3.7×10⁵/kg 之间。当移植的 GM-CFC 少于 10⁵/kg 时，粒细胞和血小板恢复时间与输注的 GM-CFC 数量之间呈反比关系。高于此剂量，移植后 10 - 15 天内出现恢复。富含干细胞的部分包含原始 T 淋巴细胞数量的 30 - 40%，并且在这些患者中有 7 例观察到了急性移植物抗宿主病。
