Rivadeneyra Almudena, Gonzalez-Martinez Alejandro, Portela Gabriela R, Martin-Ramos Daniel J, Gonzalez-Lopez Jesús, Rivadeneyra María A
Institute for Nanoelectronics, Technical University of Munich, 80333, Munich, Germany.
Department of Built Environment, School of Engineering, University of Aalto, 02150, Espoo, Finland.
Extremophiles. 2017 Nov;21(6):1049-1056. doi: 10.1007/s00792-017-0965-8. Epub 2017 Oct 10.
The ability of Halomonas maura to bioprecipitate carbonate and sulphate crystals in solid media at different manganese concentrations has been demonstrated in this study for the first time. The precipitated minerals were studied by X-ray diffraction, scanning and transmission electron microscopy, and energy-dispersive X-ray spectroscopy. The precipitated minerals were different based on the manganese concentration present in the medium and the incubation time. In the absence of manganese, H. maura formed pseudokutnahorite crystals; in the presence of manganese, the concentration in the culture medium determined the precipitation carbonates, such as rhodochrosite and dolomites. However, in the presence of low concentrations of manganese chloride (MnCl) (5 g/l), kutnohorite crystals were also formed. Finally, when H. maura was grown in the presence of manganese, small amounts of sulphate crystals (such as bassanite and gypsum) were detected. Our study of the precipitated minerals showed an active role of H. maura in the biomineralisation process, but the geochemical conditions, and the manganese concentrations in particular, were clearly influential.
本研究首次证明了嗜盐嗜碱菌在不同锰浓度的固体培养基中生物沉淀碳酸盐和硫酸盐晶体的能力。通过X射线衍射、扫描和透射电子显微镜以及能量色散X射线光谱对沉淀的矿物质进行了研究。根据培养基中存在的锰浓度和培养时间的不同，沉淀的矿物质也有所不同。在没有锰的情况下，嗜盐嗜碱菌形成了假库特钠钙石晶体；在有锰的情况下，培养基中的浓度决定了碳酸盐的沉淀，如菱锰矿和白云石。然而，在存在低浓度氯化锰（MnCl）（5 g/l）的情况下，也会形成库特钠钙石晶体。最后，当嗜盐嗜碱菌在有锰的情况下生长时，检测到少量的硫酸盐晶体（如硬石膏和石膏）。我们对沉淀矿物质的研究表明，嗜盐嗜碱菌在生物矿化过程中发挥了积极作用，但地球化学条件，尤其是锰浓度，显然具有重要影响。
