Chen Qiufang, Liang Qingfei, Zhuang Weixia, Zhou Jun, Zhang Bingyu, Xu Pu, Ju Yang, Morita Yasuyuki, Luo Qing, Song Guanbin
Key Laboratory of Biorheological Science and Technology, College of Bioengineering, Ministry of Education, Chongqing University, Chongqing, 400044, China.
Post-doctoral Mobile Stations of Biology, Chongqing University, Chongqing, 400044, China.
Biotechnol Lett. 2018 Jan;40(1):215-224. doi: 10.1007/s10529-017-2446-7. Epub 2017 Oct 10.
To investigate the impact of secreted factors of rat bone marrow mesenchymal stem cells (MSCs) on the proliferation and migration of tenocytes and provide evidence for the development of MSC-based therapeutic methods of tendon injury.
Rat bone marrow mesenchymal stem cell-derived conditioned medium (MSC-CM) promoted the proliferation of tenocytes within 24 h and decreased the percentage of tenocytes in G1 phase. MSC-CM activated the extracellular signal-regulated kinase1/2 (ERK1/2) signal molecules, while the ERK1/2 inhibitor PD98059 abrogated the MSC-CM-induced proliferation of tenocytes, decreased the fraction of tenocytes in the G1 phase and elevated p-ERK1/2 expression. Furthermore, MSC-CM promoted the migration of tenocytes within 6 h, enhanced the formation of filamentous actin (F-actin) and increased the cellular and nuclear stiffness of tenocytes.
MSC-CM promotes tenocyte proliferation by changing cell cycle distribution via the ERK1/2 signaling pathway. MSC-CM-induced tenocyte migration was accompanied by cytoskeletal polymerization and increases in cellular and nuclear stiffness.
研究大鼠骨髓间充质干细胞（MSCs）分泌因子对肌腱细胞增殖和迁移的影响，为基于MSCs的肌腱损伤治疗方法的开发提供依据。
大鼠骨髓间充质干细胞条件培养基（MSC-CM）在24小时内促进肌腱细胞增殖，并降低G1期肌腱细胞的百分比。MSC-CM激活细胞外信号调节激酶1/2（ERK1/2）信号分子，而ERK1/2抑制剂PD98059消除了MSC-CM诱导的肌腱细胞增殖，降低了G1期肌腱细胞的比例，并提高了p-ERK1/2的表达。此外，MSC-CM在6小时内促进肌腱细胞迁移，增强丝状肌动蛋白（F-肌动蛋白）的形成，并增加肌腱细胞的细胞和核硬度。
MSC-CM通过ERK1/2信号通路改变细胞周期分布促进肌腱细胞增殖。MSC-CM诱导的肌腱细胞迁移伴随着细胞骨架聚合以及细胞和核硬度的增加。
