[雄激素非依赖性前列腺癌发生发展过程中垂体瘤转化基因1的表达]
[Expression of pituitary tumor-transforming gene 1 during the development of androgen-independent prostate cancer].
作者信息
Cao Xi-Liang, Song Xiao-Ming, Yu Wen-Chao, Chen Yong-Qiang, Wei Yang-Yang, Liu Yong-Liang, Lu Ke-Quan
机构信息
Department of Urology, Huaihai Hospital Affiliated to Bengbu Medical College / The 97th Hospital of PLA, Xuzhou, Jiangsu 221004, China.
Biological Therapy Center, Huaihai Hospital Affiliated to Bengbu Medical College / The 97th Hospital of PLA, Xuzhou, Jiangsu 221004, China.
出版信息
Zhonghua Nan Ke Xue. 2016 Aug;22(8):686-691.
OBJECTIVE
To explore the expression of pituitary tumor transforming gene 1 (PTTG1) during the transformation of prostate cancer from androgen-dependent (ADPC) to androgen-independent (AIPC).
METHODS
We established an AIPC cell model LNCaP-AI by culturing the androgen-dependent LNCaP cell line in the hormone-deprived medium for over 3 months. The cell model was verified and the PTTG1 expression in the LNCaP cells was detected by Western blot and RT-PCR during hormone deprivation.
RESULTS
The AIPC cell model LNCaP-AI was successfully established. The PTTG1 expression was gradually increased in the LNCaP cells with the prolonged time of hormone deprivation and the expressions of matrix metalloproteinases MMP-2 and -9 were elevated at the same time.
CONCLUSIONS
The expression of PTTG1 is increased gradually in AIPC, which may be a target of gene therapy for advanced prostate cancer.
目的
探讨垂体瘤转化基因1（PTTG1）在前列腺癌从雄激素依赖型（ADPC）向雄激素非依赖型（AIPC）转变过程中的表达情况。
方法
通过在激素缺乏培养基中培养雄激素依赖的LNCaP细胞系3个月以上，建立AIPC细胞模型LNCaP-AI。对细胞模型进行验证，并在激素剥夺期间通过蛋白质免疫印迹法（Western blot）和逆转录-聚合酶链反应（RT-PCR）检测LNCaP细胞中PTTG1的表达。
结果
成功建立了AIPC细胞模型LNCaP-AI。随着激素剥夺时间的延长，LNCaP细胞中PTTG1的表达逐渐增加，同时基质金属蛋白酶MMP-2和MMP-9的表达也升高。
结论
PTTG1在AIPC中的表达逐渐增加，这可能是晚期前列腺癌基因治疗的一个靶点。
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