Fakouri Aris, Asghari Ahmad, Akbari Ghasem, Mortazavi Pejman
Graduate student, Science and Research Branch, Islamic Azad University, Tehran, Iran. Technical procedures, acquisition and interpretation of data.
Associate Professor, Department of Surgery, Science and Research Branch, Islamic Azad University, Tehran, Iran. Design and supervised all phases of the study, manuscript writing.
Acta Cir Bras. 2017 Sep;32(9):755-766. doi: 10.1590/s0102-865020170090000008.
To determine the effect of folic acid (FA) on experimental testicular ischemia/reperfusion (I/R) in rats.
Sixty male Wistar rats were randomly divided into 6 groups. The control group received physiologic saline orally. The sham-operated group received physiologic saline orally then exposed to midline laparotomy without clamping the IR. The I/R rats received oral gavage of the saline then subjected to 1h ischemia /24h reperfusion, period. In folic acid (2mg/kg+IR) rats received oral gavage of the FA (2mg/kg) then subjected to 1h I/24h R. groups 5-6 received FA (5 and 10 mg/kg), then subjected to 1 h I/24 h, respectively. At the end of the study, semen samples were collected for spermatozoa characteristics. The left testis was removed for histological analysis and superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GPx) measurement.
Spermatozoa mobility, mortality (%) significantly decreased in I/R group (P<0.05). Dose dependent increase observed on spermatozoa mobility, mortality (%) using different levels of the FA (2, 5 and 10 mg/kg) treated rat (P<0.05). Tissue MDA levels significantly increased in I/R rat (P<0.05) while FA (2, 5 and 10mg/kg) in a dose dependent manner decreased I/R-induced MDA (P<0.05). Experimental I/R significantly decreased SOD and GPx activity (P<0.05). Administration of the FA (2, 5 and 10mg/kg) significantly increased tissue SOD and GPx activity in I/R rat (P<0.05). Seminiferous tubules degenerated and loss of spermatogenesis with few spermatocytes was observed in degenerated testis tubules in I/R rat. Orally administration of the FA (5 and 10 mg/kg) improved testis characteristics with few normal seminiferous tubules and spermatocyte in seminiferous tubules in experimental I/R-induced rat.
The treatment of folic acid had a benefit effect against ischemia-reperfusion.
确定叶酸（FA）对大鼠实验性睾丸缺血/再灌注（I/R）的影响。
将60只雄性Wistar大鼠随机分为6组。对照组口服生理盐水。假手术组口服生理盐水，然后进行中线剖腹术但不夹闭缺血/再灌注。I/R组大鼠口服生理盐水灌胃，然后经历1小时缺血/24小时再灌注期。叶酸（2mg/kg + I/R）组大鼠口服叶酸（2mg/kg）灌胃，然后经历1小时缺血/24小时再灌注。第5 - 6组分别给予叶酸（5mg/kg和10mg/kg），然后经历1小时缺血/24小时再灌注。研究结束时，收集精液样本以检测精子特征。取出左侧睾丸进行组织学分析以及超氧化物歧化酶（SOD）、丙二醛（MDA）和谷胱甘肽过氧化物酶（GPx）的测量。
I/R组精子活力、死亡率（%）显著降低（P<0.05）。使用不同剂量叶酸（2mg/kg、5mg/kg和10mg/kg）处理的大鼠，精子活力、死亡率（%）呈剂量依赖性增加（P<0.05）。I/R组大鼠组织MDA水平显著升高（P<0.05），而叶酸（2mg/kg、5mg/kg和10mg/kg）以剂量依赖性方式降低I/R诱导的MDA（P<0.05）。实验性I/R显著降低SOD和GPx活性（P<0.05）。给予叶酸（2mg/kg、5mg/kg和10mg/kg）显著提高I/R组大鼠组织SOD和GPx活性（P<0.05）。在I/R组大鼠退化的睾丸小管中观察到生精小管退化和生精过程丧失，伴有少量精母细胞。口服叶酸（5mg/kg和10mg/kg）改善了实验性I/R诱导大鼠的睾丸特征，生精小管中有少量正常生精小管和精母细胞。
叶酸治疗对缺血再灌注具有有益作用。
