Orozco E, Solís F J, Domínguez J, Chávez B, Hernández F
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México, D.F.
Exp Parasitol. 1988 Oct;67(1):85-95. doi: 10.1016/0014-4894(88)90011-2.
The cell cycle of Entamoeba histolytica, the duration of its phases, and the details of the nuclear division stages are described in this paper. Trophozoites from clone L-6, strain HM1:IMSS, were synchronized by colchicine. Synchrony was observed immediately after treatment and cultures remained synchronous for at least three replicative cycles with synchrony indexes between 13 and 15 hr. The stages of nuclear division were studied by light and electron microscopy. Four stages of the nuclear division were defined: prophase, early anaphase, late anaphase, and telophase. No metaphase stage was observed by light or electron microscopy. One of the first events in the nuclear division was the presence of a bud close to the juxtanuclear body, which grew to a daughter nucleus. The karyosome and the nuclear membrane remained throughout the mitotic process. Bundles of intranuclear microtubules were observed forming a "V" from the center of the nucleus to one of the poles, and associated with them, 12 to 16 chromosomes-like structures appeared. The results of these studies strongly suggest that division of E. histolytica involved a pleuromitotic process which is carried out in about 120 min.
本文描述了溶组织内阿米巴的细胞周期、各阶段的持续时间以及核分裂阶段的细节。来自克隆L - 6、菌株HM1:IMSS的滋养体用秋水仙碱进行同步化处理。处理后立即观察到同步化现象，培养物在至少三个复制周期内保持同步，同步指数在13至15小时之间。通过光学显微镜和电子显微镜研究核分裂阶段。定义了核分裂的四个阶段：前期、早后期、晚后期和末期。光学显微镜和电子显微镜均未观察到中期阶段。核分裂的最早事件之一是在近核体附近出现一个芽，该芽发育成子核。核小体和核膜在整个有丝分裂过程中一直存在。观察到核内微管束从细胞核中心向其中一个极形成一个“V”形，与之相关联的是，出现了12至16个染色体样结构。这些研究结果有力地表明，溶组织内阿米巴的分裂涉及一个大约在120分钟内完成的多极有丝分裂过程。
