Boyer R, Tapia-Arancibia L, Alonso G, Arancibia S
Laboratoire de Physiologie, Faculté de Chirurgie Dentaire, Université de Montpellier I.
J Biol Buccale. 1988 Jun;16(2):69-74.
Indirect relationships are thought to exist between submandibular salivary glands (SSG) and the central nervous system (CNS) via superior cervical ganglia (SCG). To study this topic, the concentrations of thyrotropin releasing hormone (THR) and somatostatin (SRIF) were measured in both whole and specific areas of the hypothalamus, as well as plasmatic thyrotropin stimulating hormone (TSH) levels following ablation of SSG. Twenty, forty and fifty days after ablation of SSG, groups of operated and sham-operated animals weighing 230-260 g at the beginning of experimentation, were killed by cervical dislocation. Plasma was taken, frozen and stored for TSH-radioimmunoassay (RIA) and the hypothalami were removed and homogenized in either 0.1 N HCl (for TRH-RIA) or 0.2 N acetic acid (for SRIF-RIA). Twenty days after ablation of SSG in another group, TRH concentrations were measured in both the median eminence (ME) and the paraventricular nucleus (PVN), dissected by the micropunch technique. The results show that twenty days after SSG ablation, the hypothalamic TRH concentrations was significantly lower in operated than in sham-operated animals (295.2 +/- 24.8 vs 226 +/- 11.15 pg/mg hypothalamus respectively p less than 0.01, n = 9). No differences were observed at later intervals. This finding seems to be specific for this peptide since the SRIF level was not modified twenty days after SSG removal. Among the discrete hypothalamic areas examined, only the ME exhibited a significant decrease in TRH content (25.43 +/- 3.02 ng/mg prot. VS. 41.24 +/- 1.33 ng/mg prot., respectively). Despite these results on TRH levels, no modifications in plasmatic TSH levels were observed.(ABSTRACT TRUNCATED AT 250 WORDS)
人们认为下颌下唾液腺（SSG）与中枢神经系统（CNS）之间通过颈上神经节（SCG）存在间接联系。为研究这一课题，在切除SSG后，测量了下丘脑整体及特定区域促甲状腺激素释放激素（THR）和生长抑素（SRIF）的浓度，以及血浆促甲状腺激素（TSH）水平。在切除SSG后的第20、40和50天，将实验开始时体重230 - 260克的手术组和假手术组动物通过颈椎脱臼法处死。采集血浆，冷冻保存用于TSH放射免疫分析（RIA），取出下丘脑并在0.1N盐酸（用于TRH - RIA）或0.2N乙酸（用于SRIF - RIA）中匀浆。在另一组SSG切除后20天，通过微穿孔技术分离出正中隆起（ME）和室旁核（PVN），测量其中TRH浓度。结果显示，SSG切除后20天，手术组动物下丘脑TRH浓度显著低于假手术组（分别为295.2±24.8与226±11.15 pg/mg下丘脑，p＜0.01，n = 9）。在随后的时间段未观察到差异。这一发现似乎对该肽具有特异性，因为SSG切除20天后SRIF水平未改变。在所检查的离散下丘脑区域中，只有ME的TRH含量显著降低（分别为25.43±3.02 ng/mg蛋白对41.24±1.33 ng/mg蛋白）。尽管有这些关于TRH水平的结果，但未观察到血浆TSH水平的改变。（摘要截断于250字）
