Ulčová-Gallová Z, Pešek M, Chaloupka P, Mukešnábl P, Haschová M, Losan P, Bibková K, Mičanová Z, Cibulka J
Ceska Gynekol. 2017 Fall;82(5):366-371.
Generaly, natural killer cells (NK cells) are among the most important cells of our immune defense system. They are present in the blood, decidua but also in secretory endometrium.
We investigate an association between high density of NK cells CD56+ and NK cells CD16+ into blood and secretory endometrium in patients with up to now unexplained recurrent miscarriage. At the same time, we focused on diagnosis of antiphospholipid syndrome, congenital trombophilic factors, stress factor, and lenght of hormonal contraception before conception.
Original work-prospective study.
Genetics-Pilsen.
We investigated 59 patients aged 25-41 (average 34) years with their history of 3-9 abortions. We monitored their blood cells by flow cytometry and endometrium obtained by hysteroscopy performed on the 22nd-24th day of the menstrual cycle. We concentrated on the cellular immunity focused on the identification of lymphocytes CD56+ and CD16+. ELISA method was used for identification of antiphospholipid antibodies.
Our study goes on, we are publishing our preliminary results. We found a high density of endometrial NK cells CD56+ in 41/57, NK cells CD16+ in 40/57, both NK cells CD56+ and CD16+ in 36/57 patients. In 13 women, we identified only sporadically these cells in the secretory endometrium. NK cells CD56+ and NK cells CD16+ in the blood were all normal number in all our patients. Three of them have a homozygot form of Leiden mutation, and 18/57 primary antiphospholipid syndrome, 12 women have university education, four of them, medical doctors, have regular night services in the hospital.
Overproduction of endometrial NK cells is associated with increased local activity of embryocytotoxic cytokines that may negatively affect pregnancy. Treatment of patients with pathological immunological findings must be solved individually, as we will show soon in part two of our study.
一般来说，自然杀伤细胞（NK细胞）是我们免疫防御系统中最重要的细胞之一。它们存在于血液、蜕膜中，也存在于分泌期子宫内膜中。
我们研究不明原因复发性流产患者血液和分泌期子宫内膜中CD56⁺NK细胞和CD16⁺NK细胞的高密度之间的关联。同时，我们关注抗磷脂综合征、先天性血栓形成倾向因素、应激因素以及受孕前激素避孕的时长的诊断。
原创性工作——前瞻性研究。
皮尔森遗传学中心。
我们调查了59名年龄在25至41岁（平均34岁）、有3至9次流产史的患者。我们通过流式细胞术监测他们的血细胞，并在月经周期的第22至24天通过宫腔镜检查获取子宫内膜。我们专注于以识别CD56⁺和CD16⁺淋巴细胞为重点的细胞免疫。采用ELISA法鉴定抗磷脂抗体。
我们的研究仍在进行中，现公布初步结果。我们发现57例患者中有41例子宫内膜CD56⁺NK细胞高密度，40例CD16⁺NK细胞高密度，36例患者CD56⁺和CD16⁺NK细胞均高密度。在13名女性中，我们在分泌期子宫内膜中仅偶尔发现这些细胞。我们所有患者血液中的CD56⁺NK细胞和CD16⁺NK细胞数量均正常。其中3人有纯合子形式的莱顿突变，57例中有18例患有原发性抗磷脂综合征，12名女性拥有大学学历，其中4名是医生，在医院定期值夜班。
子宫内膜NK细胞的过度产生与胚胎细胞毒性细胞因子的局部活性增加有关，这可能对妊娠产生负面影响。对于有病理免疫学发现的患者的治疗必须个体化解决，正如我们将在研究的第二部分中很快展示的那样。
