Hamlet R, Hopewell J W
CRC Normal Tissue Radiobiology Research Group, Research Institute (University of Oxford), Churchill Hospital, U.K.
Int J Radiat Biol. 1988 Oct;54(4):675-82. doi: 10.1080/09553008814552101.
Investigations were carried out into the time- and dose-related changes in the density of fibroblasts in the dermis of irradiated pig skin. The time course of these changes in the density of fibroblast nuclei in the reticular dermis was studied from 6 to 104 weeks after irradiation with a single dose of 15.4 Gy of X-rays. The largest decrease in the number of fibroblasts occurred between 12 weeks and 26 weeks after irradiation; after this time there was only a slight fall in the fibroblast number until 104 weeks when the observations ceased. At 26 weeks and later times after irradiation the reduction in the density of fibroblast nuclei in the reticular dermis was dose-dependent for single doses in the range 8.0-20.7 Gy. The dose-response curve had an initial shoulder, after which the fall in the fibroblast nuclear density was linearly related to dose. Data obtained at other times, between 26 weeks and 104 weeks after irradiation, could be fitted by the same dose-response curve. The fall in the counts of fibroblast nuclei was compared with earlier studies in pig skin. The loss of fibroblasts occurred after an initial reduction in blood flow in the pig skin but was concomitant with the general reduction in dermal thickness.
对受辐照猪皮肤真皮中成纤维细胞密度的时间和剂量相关变化进行了研究。在用15.4 Gy单次剂量X射线辐照后6至104周内，研究了网状真皮中成纤维细胞核密度这些变化的时间进程。成纤维细胞数量的最大减少发生在辐照后12周和26周之间；在此之后，成纤维细胞数量仅略有下降，直至104周观察结束。在辐照后26周及更晚时间，对于8.0 - 20.7 Gy范围内的单次剂量，网状真皮中成纤维细胞核密度的降低呈剂量依赖性。剂量反应曲线有一个初始平台期，在此之后成纤维细胞核密度的下降与剂量呈线性相关。在辐照后26周和104周之间的其他时间获得的数据可以用相同的剂量反应曲线拟合。将成纤维细胞核计数的下降与猪皮肤早期研究进行了比较。成纤维细胞的损失发生在猪皮肤血流最初减少之后，但与真皮厚度的普遍减少同时出现。
