Mu Huawei, Sun Jin, Heras Horacio, Chu Ka Hou, Qiu Jian-Wen
Department of Biology, Hong Kong Baptist University, Hong Kong, China.
Division of Life Science, Hong Kong University of Science and Technology, Hong Kong, China.
Data Brief. 2017 Sep 22;15:203-207. doi: 10.1016/j.dib.2017.09.020. eCollection 2017 Dec.
This article describes how the proteomic and transcriptomic data were produced during a study of the reproductive proteins of , an aquatic apple snail laying colorful aerial eggs, and provides public access to the data. The data are related to a research article titled 'An integrated proteomic and transcriptomic analysis of perivitelline fluid proteins in a freshwater gastropod laying aerial eggs' (Mu et al., 2017) [1]. RNA was extracted from the albumen gland and other tissues and sequenced on an Illumina Hiseq. 2000. The assembled transcriptome was translated into protein sequences and then used for protein identification. Proteins from the perivitelline fluid of were separated in SDS-PAGE and analyzed by LTQ-Orbitrap Elite coupled to an Easy-nLC. The translated transcriptome data are provided in this article. Proteomic data (. file format) are available via ProteomeXchange with the identifier PXD006718.
本文描述了在对一种产下彩色气生卵的水生苹果螺的生殖蛋白进行研究期间，蛋白质组学和转录组学数据是如何产生的，并提供了数据的公共访问途径。这些数据与一篇题为《对产气生卵的淡水腹足动物卵周液蛋白的蛋白质组学和转录组学综合分析》的研究文章相关（Mu等人，2017年）[1]。从蛋白腺和其他组织中提取RNA，并在Illumina Hiseq. 2000上进行测序。组装好的转录组被翻译成蛋白质序列，然后用于蛋白质鉴定。该苹果螺卵周液中的蛋白质在SDS-PAGE中进行分离，并通过与Easy-nLC联用的LTQ-Orbitrap Elite进行分析。本文提供了翻译后的转录组数据。蛋白质组学数据（.文件格式）可通过蛋白质组交换库获得，标识符为PXD006718。
