Kato Y, Sawada H, Tashima M, Yumoto Y, Okuda T, Ueda T, Uchino H, Hotta T, Mitsutani S, Ono T
First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Leukemia. 1988 Oct;2(10):701-3.
We surveyed DNAs from patients with various hematological malignancies by Southern blot hybridization to analyze bcr rearrangements, and detected a new restriction fragment length polymorphism (RFLP) of the breakpoint cluster region at a BamHI site in three patients. By using a 1.2-kb HindIII-BglII 3' bcr probe, unusual BamHI restriction enzyme fragments (1.9 kb and 1.4 kb) were detected from the DNAs of three patients with hematological malignancies. DNAs from cultured fibroblasts derived from the skin of a patient, as well as from peripheral leukocytes of the father of a patient and the mother of another patient, showed identical 1.9- and 1.4-kb additional bands, besides a 3.3-kb germline band, establishing that polymorphism, rather than gene arrangement, was responsible for these additional restriction enzyme fragments. However, RFLP was not detected in the DNAs of 40 normal unrelated individuals.
我们通过Southern印迹杂交对各种血液系统恶性肿瘤患者的DNA进行了检测，以分析bcr重排，并在三名患者的BamHI位点检测到了断裂点簇区域的一种新的限制性片段长度多态性（RFLP）。使用一个1.2 kb的HindIII-BglII 3'bcr探针，从三名血液系统恶性肿瘤患者的DNA中检测到了异常的BamHI限制性酶切片段（1.9 kb和1.4 kb）。来自一名患者皮肤的培养成纤维细胞的DNA，以及一名患者父亲和另一名患者母亲的外周血白细胞的DNA，除了3.3 kb的种系条带外，还显示出相同的1.9 kb和1.4 kb的额外条带，这表明这些额外的限制性酶切片段是由多态性而非基因重排导致的。然而，在40名正常无关个体的DNA中未检测到RFLP。
