Dano Ibrahim Dan, Sadou Hassimi, Issaka Bassira, Oukem-Boyer Odile Ouwe Missi
Centre de Recherche Medicale et Sanitaire (CERMES), 634 Boulevard de la Nation YN034, BP 10887, Niamey, Niger.
Faculte des Sciences et Techniques, Universite Abdou Moumouni, BP 10 662, Niamey, Niger.
Pak J Biol Sci. 2016;19(4):185-190. doi: 10.3923/pjbs.2016.185.190.
It is assessed whether the measurement of interleukin-6 in the cerebrospinal fluid can serve as a biomarker for the diagnosis of bacterial meningitis.
Cerebrospinal fluid was obtained from 152 patients aged 0-15 years suspected of having meningitis. These patients were classified into the following groups: Bacterial meningitis (n = 85), aseptic meningitis (n = 35) and non-meningitis/control (n = 32) based on leukocyte count and bacterial identification by culture and molecular biology. Interleukin-6 concentrations in cerebrospinal fluid were measured by enzyme-linked immunosorbent assay.
This study found a significant difference of the mean cerebrospinal fluid interleukin-6 level (p≤0.01) between patients with bacterial meningitis (3,538.69±2,560.78 pg mL -1) and patients with aseptic meningitis (332.51±470.69 pg mL -1) or those of the control group (205.83±79.39 pg mL -1). There was also a significant difference of the mean cerebrospinal fluid interleukin-6 level between patients with aseptic meningitis and those of the control group. Interleukin-6 had the highest area under the ROC curve: 0.94 (95% confidence interval: 0.901-0.979) compared to that of cerebrospinal fluid glucose and total protein. At a cut-off value of 1,065.96 pg mL -1, interleukin-6 had a sensitivity of 76.2% and specificity of 100%.
Interleukin-6 is a potential biomarker for the differential diagnosis of meningitis.
评估脑脊液中白细胞介素-6的测量是否可作为细菌性脑膜炎诊断的生物标志物。
从152例年龄在0至15岁疑似患有脑膜炎的患者中获取脑脊液。根据白细胞计数以及通过培养和分子生物学进行的细菌鉴定，将这些患者分为以下几组：细菌性脑膜炎（n = 85）、无菌性脑膜炎（n = 35）和非脑膜炎/对照组（n = 32）。采用酶联免疫吸附测定法测量脑脊液中白细胞介素-6的浓度。
本研究发现，细菌性脑膜炎患者（3,538.69±2,560.78 pg/mL -1）与无菌性脑膜炎患者（332.51±470.69 pg/mL -1）或对照组患者（205.83±79.39 pg/mL -1）之间，脑脊液白细胞介素-6平均水平存在显著差异（p≤0.01）。无菌性脑膜炎患者与对照组患者之间的脑脊液白细胞介素-6平均水平也存在显著差异。与脑脊液葡萄糖和总蛋白相比，白细胞介素-6的ROC曲线下面积最高：0.94（95%置信区间：0.901 - 0.979）。在截断值为1,065.96 pg/mL -1时，白细胞介素-6的敏感性为76.2%，特异性为100%。
白细胞介素-6是脑膜炎鉴别诊断的潜在生物标志物。
