El-Ashry Amal Abd El-Latif, Gabr Ahmed Mohamed Magdy, Bekheet Shawky Abd El-Hamid
Department of Plant Biotechnology, National Research Centre, 33 El Bohouth St. Dokki, Giza, P.O. 12622, Egypt.
Pak J Biol Sci. 2017;20(7):355-364. doi: 10.3923/pjbs.2017.355.364.
Jojoba is a promising industrial plant, which recommended with pharmaceutical benefits. The present study was conducted to stimulate embryogenic calli formation from jojoba using zeatin and thidiazuron (TDZ), as well as determination of the antioxidant activity of proliferated calli.
For callus induction, leaf and stem explants derived from in vitro grown shootlets, were cultured on Murashige and Skoog (MS) medium with different combinations of 0.5 mg L-1 benzyl adenine (BA) or kinetin with 2,4-Dichlorophenoxyacetic acid (2,4-D), Naphthalene acetic acid (NAA) and picloram at 0.5 or 1mg L-1. To stimulate embryogenic calli, friable callus were transferred to woody plant medium (WPM) supplemented with different concentrations of zeatin or TDZ. Antioxidant activity of different treatments was determined using hexane or petroleum ether extraction. Data was analyzed as mean±standard deviation (SD).
The MS medium supplemented with 0.5 mg L-1 BA+0.5 or 1 mg L-1 picloram was the best treatment to obtain friable calli from both explants types. WPM medium supplemented with 2 mg L-1 zeatin gave the highest percentage of embryogenic calli derived from leaf explants. While the highest percentage of embryogenic calli derived from stem explants was registered using 1 or 4 mg L-1 TDZ containing medium. Embryogenic calli originated from leaves explants on 1.5 mg L-1 zeatin showed promising activity of antioxidant with hexane extraction. However, embryogenic calli originated from stem explants on 1 mg L-1 TDZ showed the highest antioxidant activity with petroleum ether extraction.
TDZ has promising effect on embryogenic callus induction from stem explants. While, zeatin has promising effect on embryogenic callus induction from leaf explants.
霍霍巴是一种很有前景的工业植物，具有药用价值。本研究旨在利用玉米素和噻苯隆（TDZ）诱导霍霍巴胚性愈伤组织的形成，并测定增殖愈伤组织的抗氧化活性。
为诱导愈伤组织，将体外培养的嫩枝上的叶片和茎段外植体接种在添加了不同组合植物生长调节剂的Murashige和Skoog（MS）培养基上，这些组合包括0.5 mg L-1苄基腺嘌呤（BA）或激动素与0.5或1 mg L-1的2,4-二氯苯氧乙酸（2,4-D）、萘乙酸（NAA）和毒莠定。为刺激胚性愈伤组织的形成，将易碎愈伤组织转移到添加了不同浓度玉米素或TDZ的木本植物培养基（WPM）上。使用己烷或石油醚萃取法测定不同处理的抗氧化活性。数据以平均值±标准差（SD）进行分析。
添加0.5 mg L-1 BA + 0.5或1 mg L-1毒莠定的MS培养基是从两种外植体类型获得易碎愈伤组织的最佳处理方法。添加2 mg L-1玉米素的WPM培养基诱导叶片外植体产生胚性愈伤组织的比例最高。而使用含1或4 mg L-1 TDZ的培养基诱导茎段外植体产生胚性愈伤组织的比例最高。用己烷萃取法时，来源于叶片外植体且添加1.5 mg L-1玉米素的胚性愈伤组织显示出良好的抗氧化活性。然而，用石油醚萃取法时，来源于茎段外植体且添加1 mg L-1 TDZ的胚性愈伤组织显示出最高的抗氧化活性。
TDZ对从茎段外植体诱导胚性愈伤组织有显著效果。而玉米素对从叶片外植体诱导胚性愈伤组织有显著效果。
