Berbari Roula, Fayyad-Kazan Hussein, Ezzedine Mohamad, Fayyad-Kazan Mohammad, Bandon Daniel, Sfeir Elia
Department of Pediatric Dentistry, Faculty of Dental Medicine, Lebanese University, Beirut, Lebanon.
Department of Biology, Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences-1, Lebanese University, Beirut, Lebanon.
J Int Soc Prev Community Dent. 2017 Sep-Oct;7(5):272-278. doi: 10.4103/jispcd.JISPCD_267_17. Epub 2017 Sep 18.
The aim of this study was to assess the correlation between the remaining dentin thickness (RDT) in deep decayed primary molars and the inflammatory status and bacterial composition of the corresponding coronal pulp. We hypothesized that RDT could be used as a reference for clinicians in assigning the indication for pulpotomy.
Pulpotomies were conducted on the cameral pulp of 48 primary molars. Microorganisms, such as sp., sp., and sp., were identified and quantified and levels of tumor necrosis factor-alpha (TNF-α) and interlukin-6 (IL-6) were assessed. The correlation between the pre-operative RDT based on radiographic images and inflammatory-microbial profiles was evaluated using Spearman's rho correlation coefficient. All data analysis was performed using a statistical software program (SPSS 20.0, SPSS Inc., Chicago, IL, USA).
Immunological and microbiological studies revealed elevated levels of TNF-α and IL-6 cytokines, and sp., sp. and sp. in the cameral pulp with an RDT measuring up to 1.1 mm. No significant relationship could be established between RDT, inflammatory status and microbial content of the pulps.
The RDT remains a key clinical factor that needs to be assessed when establishing the indication for pulpotomy. Additional parameters that can improve this therapy should be investigated in the future.
本研究旨在评估深度龋坏乳磨牙的剩余牙本质厚度（RDT）与相应冠髓的炎症状态和细菌组成之间的相关性。我们假设RDT可作为临床医生确定牙髓切断术适应证的参考依据。
对48颗乳磨牙的冠髓进行牙髓切断术。鉴定并定量了诸如某某菌、某某菌和某某菌等微生物，并评估了肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的水平。使用Spearman等级相关系数评估基于X线影像的术前RDT与炎症-微生物谱之间的相关性。所有数据分析均使用统计软件程序（SPSS 20.0，美国伊利诺伊州芝加哥市SPSS公司）进行。
免疫学和微生物学研究显示，在RDT高达1.1毫米的冠髓中，TNF-α和IL-6细胞因子以及某某菌、某某菌和某某菌的水平升高。在RDT、牙髓的炎症状态和微生物含量之间未能建立显著关系。
RDT仍然是确定牙髓切断术适应证时需要评估的关键临床因素。未来应研究可改善该治疗方法的其他参数。
