Tran Khoa D, Li Yan, Holiman Jeffrey D, Tang Maolong, Huang David, Straiko Michael D, Stoeger Christopher G
Vision Research Laboratory, Lions VisionGift, .
Center for Ophthalmic Optics and Lasers, Casey Eye Institute, Oregon Health and Science University.
Cell Tissue Bank. 2018 Mar;19(1):19-25. doi: 10.1007/s10561-017-9666-x. Epub 2017 Oct 12.
Irradiated corneal tissues have been used for a variety of ophthalmic procedures including glaucoma drainage device covers and lamellar grafts. The maintenance of corneal clarity is important, as light obstructions resulting from processing or long-term storage of irradiated corneas may negatively affect vision and postoperative cosmesis. It has been reported that corneal tissues can be preserved in human serum albumin (HSA), however, the clarity of corneas after long-term storage in HSA has not been well described. Furthermore, the use of donor-pooled serum increases the risk for transmission of blood-borne diseases and may induce an immune response in the recipient. Here, we examined changes in corneal clarity due to electron-beam (e-beam) irradiation and storage in a recombinant human serum albumin (rHSA). Dark-field microscopy was employed to examine the light scattering effects of fresh and irradiated corneas. Compared to measurements taken prior to tissue preparation and e-beam treatment, irradiated corneas showed an average 2.6% increase in light scattering (P = 0.002). Irradiated corneas stored in rHSA at room-temperature for 20 months showed an average increase of 11.6% light scattering compared to fresh corneas (P ≪ 0.01), but did not negatively affect the visualization of printed text, and were deemed suitable for transplant use. Therefore, the slight increase in cornea light scattering, and resulting reduction in corneal clarity, after e-beam treatment and long-term storage in rHSA may not be clinically significant. These results suggest that e-beam sterilized corneal grafts may be used as an alternative to fresh tissue for certain ophthalmic applications.
经辐照的角膜组织已被用于多种眼科手术，包括青光眼引流装置覆盖物和板层移植。维持角膜透明度很重要，因为辐照角膜的加工或长期储存导致的光线阻挡可能会对视力和术后美观产生负面影响。据报道，角膜组织可以保存在人血清白蛋白（HSA）中，然而，长期保存在HSA中的角膜透明度尚未得到充分描述。此外，使用供体混合血清会增加血源性疾病传播的风险，并可能在受者中引发免疫反应。在此，我们研究了电子束（e-beam）辐照和在重组人血清白蛋白（rHSA）中储存导致的角膜透明度变化。采用暗场显微镜检查新鲜角膜和辐照角膜的光散射效应。与组织制备和电子束处理前的测量结果相比，辐照角膜的光散射平均增加了2.6%（P = 0.002）。在室温下于rHSA中储存20个月的辐照角膜与新鲜角膜相比，光散射平均增加了11.6%（P ≪ 0.01），但并未对打印文本的可视性产生负面影响，被认为适合移植使用。因此，电子束处理和在rHSA中长时间储存后角膜光散射的轻微增加以及由此导致的角膜透明度降低可能在临床上并不显著。这些结果表明，电子束灭菌的角膜移植物可作为某些眼科应用中新鲜组织的替代品。
