雌激素在长期培养中保留人牙周膜干细胞的干性。
Oestrogen retains human periodontal ligament stem cells stemness in long-term culture.
作者信息
Ou Qianmin, Wang Xiaoxiao, Wang Yanlan, Wang Yan, Lin Xuefeng
机构信息
Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
Faculty of Dentistry, The University of Hong Kong, Hong Kong, SAR, China.
出版信息
Cell Prolif. 2018 Apr;51(2):e12396. doi: 10.1111/cpr.12396. Epub 2017 Oct 12.
OBJECTIVES
During long-term culture, loss of stemness is observed which greatly restricts the application of human periodontal ligament stem cells (hPDLSCs) in tissue regeneration. Oestrogen (E2) was found to significantly enhance the proliferation and osteogenic differentiation capacity in mesenchymal stem cells. Therefore, in this study, we investigated effects of E2 on hPDLSCs stemness in long-term culture.
MATERIALS AND METHODS
Effects of E2 on hPDLSCs stemness were systematically evaluated. To characterize underlying the mechanisms, its effects on PI3K/AKT signalling pathway were determined.
RESULTS
Our results showed that E2 was able to enhance the proliferation, modify cell cycle, up-regulate stemness-related genes expression, promote osteogenic differentiation and elevate the positive rate of CD146 and STRO-1 over 10 passages in hPDLSCs. Importantly, PI3K/AKT signing pathway might play a role in these effects.
CONCLUSIONS
These findings suggest that E2 retains hPDLSCs stemness in long-term culture, which might enhance its application in tissue engineering.
目的
在长期培养过程中，会观察到干性丧失，这极大地限制了人牙周膜干细胞（hPDLSCs）在组织再生中的应用。已发现雌激素（E2）可显著增强间充质干细胞的增殖和成骨分化能力。因此，在本研究中，我们调查了E2对长期培养的hPDLSCs干性的影响。
材料与方法
系统评估E2对hPDLSCs干性的影响。为了阐明潜在机制，确定其对PI3K/AKT信号通路的影响。
结果
我们的结果表明，E2能够增强hPDLSCs的增殖、改变细胞周期、上调干性相关基因表达、促进成骨分化，并在超过10代培养中提高CD146和STRO-1的阳性率。重要的是，PI3K/AKT信号通路可能在这些作用中发挥作用。
结论
这些发现表明，E2在长期培养中保留了hPDLSCs的干性，这可能会增强其在组织工程中的应用。
Zotero 插件