pNDM-BTR 中携带 In191 和 Tn6360 以及其他 bla 基因的 IncN1 质粒的结构基因组学。
Structural genomics of pNDM-BTR harboring In191 and Tn6360, and other bla -carrying IncN1 plasmids.
机构信息
State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
Department of Laboratory Medicine, Beijing Tsinghua Chang Gung Hospital, Tsinghua University, Beijing 102218, China.
出版信息
Future Microbiol. 2017 Nov;12:1271-1281. doi: 10.2217/fmb-2017-0067. Epub 2017 Oct 13.
AIM
To characterize a conjugative bla -carrying plasmid pNDM-BTR from a clinical Escherichia coli isolate.
MATERIALS & METHODS: The complete nucleotide sequence of pNDM-BTR was determined using next-generation sequencing technology. Comparative genomic analysis of bla -carrying IncN1 plasmids, including pNDM-BTR, was performed, and the antimicrobial resistance phenotypes were determined.
RESULTS
pNDM-BTR contained three accessory modules, namely IS26, a novel Tn3-family transposon Tn6360 and the dfrA14 region composed of In191, ecoRII-ecoRIImet and ΔIS1X2. The relatively small IncN1 backbones could integrate massive accessory modules, most of which were integrated at two 'hotspots'. These IncN1 plasmids contained distinct profiles of accessory modules, which included those carrying various resistance genes.
CONCLUSION
This study provides a deeper insight into horizontal transfer of resistance genes among IncN1 plasmids.
目的
对临床分离的大肠杆菌携带 bla 的可接合质粒 pNDM-BTR 进行特征分析。
材料与方法
采用新一代测序技术确定 pNDM-BTR 的完整核苷酸序列。对包括 pNDM-BTR 在内的 bla 携带 IncN1 质粒进行比较基因组分析，并确定其抗生素耐药表型。
结果
pNDM-BTR 包含三个辅助模块，即 IS26、新型 Tn3 家族转座子 Tn6360 和由 In191、ecorII-ecorIImet 和 ΔIS1X2 组成的 dfrA14 区。相对较小的 IncN1 骨架可以整合大量辅助模块，其中大多数整合在两个“热点”上。这些 IncN1 质粒包含不同的辅助模块特征，包括携带各种耐药基因。
结论
本研究深入了解了 IncN1 质粒之间耐药基因的水平转移。
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